新型发热伴血小板减少综合征病毒的制作方法

本发明涉及新型基因型发热伴血小板减少综合征病毒及其免疫原性组合物的用途。

背景技术：

发热伴血小板减少综合征(severefeverwiththrombocytopeniasyndrome，sfts)是一种伴随着如高烧、呕吐、腹泻、血小板减少、白细胞减少及多发性器官功能衰竭等症状，死亡率高达6～30％的严重的疾病(yuxjetal.，n.engl.j.med.2011；364:1523-32；dingfetalclininfectdis2013；56:1682-3)。

发热伴血小板减少综合征的原因病原体为发热伴血小板减少综合征病毒(severefeverthrombocytopeniasyndromevirus，sftsv)，属于布尼亚病毒科(bunyaviridae)。布尼亚病毒科为包含三个节段的负链(negative-strand)rna病毒，由包括正布尼病毒(orthobunyavirus)，汉他病毒(hantavirus)，内罗病毒(nairovirus)，白蛉病毒(phlebovirus)，番茄斑萎病毒(tospovirus)的五个属(genus)形成。发热伴血小板减少综合征病毒属于白蛉病毒属(phlebovirusgenus)，裂谷热病毒(riftvalleyfevervirus)等就属于这种病毒属中。2011年在中国首次报道过发热伴血小板减少综合征病毒(yuxjetal.ibid)，这种病毒为在除了中国之外的韩国和日本持续发病的新变种病毒。发热伴血小板减少综合征病毒为直径为80～100nm的球形病毒。此病毒具有作为单链负股rna节段(single-strandednegativesensernasegment)的大节段(large(l)segment)、中节段(medium(m)segment)及小节段(small(s)segment；np，ns)等三个基因。

发热伴血小板减少综合征病毒以长角血蜱(haemaphysalislongicornis)作为媒介，上述蜱也遍布于韩国(chaejsetal.jvetsci2008；9:285-93；kimcmetal.applenvironmicrobiol2006；72:5766-76)。发热伴血小板减少综合征病毒的血清转换及病毒血症被发现于山羊、羊、猪及狗等饲养动物中，而这种动物也在发热伴血小板减少综合征病毒分布的地区中作用为中间媒介(zhaoletal.emerginfectdis2013；18:963-5；niugetal.emerginfectdis2013；19:756-63)。在患者的血液中检测到发热伴血小板减少综合征病毒，尤其在重症患者的血液中的发热伴血小板减少综合征病毒的浓度非常高，因此，人与人之间可通过血液传播(tangx，wuw，wangh，etal.jinfectdis2013；207:736-739.)。

由于尚未开发出针对发热伴血小板减少综合征病毒的抗病毒剂，对于发热伴血小板减少综合征的治疗，主要使用输血、肾替代疗法等针对器官功能衰竭的保守疗法。在中国，从2012年开始将利巴韦林静脉注射导入了治疗方针上，在最近发表的治疗结果中，利巴韦林给药组和非给药组之间的死亡率并不存在差异。因此，需要针对发热伴血小板减少综合征病毒的疫苗，但至今还未开发出疫苗。

技术实现要素：

技术问题

本发明的目的在于，提供新型基因型发热伴血小板减少综合征病毒及利用其的免疫原性组合物。

技术方案

为了实现上述目的，本发明提供新型发热伴血小板减少综合征病毒。

并且，本发明提供用于预防或治疗发热伴血小板减少综合征的免疫原性组合物。

并且，本发明提供针对发热伴血小板减少综合征病毒或其抗原的抗体。

并且，本发明提供发热伴血小板减少综合征病毒的诊断试剂盒。

并且，本发明提供发热伴血小板减少综合征病毒抗体的检测方法。

并且，本发明提供生产针对发热伴血小板减少综合征病毒的抗血清的方法。

同时，本发明提供与发热伴血小板减少综合征的诊断有关的信息的提供方法。

有益效果

本发明的发热伴血小板减少综合征病毒与以往的血小板减少综合征病毒具有遗传性区别，是在系统学上从b基因型细化的新型病毒，因此，在仅通过某个特定基因型的病毒产生限制性防御能力的疫苗的特性上，可将本发明的新型病毒有用地利用为针对发热伴血小板减少综合征病毒具有优秀的交叉免疫原性的疫苗。

附图说明

图1为示出本发明的三种新型病毒和在本发明的三种新型病毒分离的发热伴血小板减少综合征病毒l基因的系统树图的图。

图2为示出本发明的三种新型病毒和在本发明的三种新型病毒分离的发热伴血小板减少综合征病毒m基因的系统树图的图。

图3为示出本发明的三种新型病毒和在本发明的三种新型病毒分离的发热伴血小板减少综合征病毒s(np)基因的系统树图的图。

图4为示出本发明的三种新型病毒和在本发明的三种新型病毒分离的发热伴血小板减少综合征病毒s(ns)基因的系统树图的图。

图5为示出本发明的b-1(cb3/2016)、b-2(cb7/2017)及b-3(cb6/2016)基因型的三种新型病毒的l基因及m基因中的氨基酸变异部位的图。

图6为确认本发明的b-1(cb3/2016)、b-2(cb7/2017)及b-3(cb6/2016)基因型的三种新型病毒的疫苗效果的图。

最优实施方式

以下，参照附图通过本发明的实例更加详细地说明。但是，以下实例为了例示本发明而揭示，当本领域的普通技术人员判断主旨显要的描述或对结构的具体说明不必要地混淆本发明的要旨时，可省略其详细说明，而本发明并不限定于此。本发明在发明要求保护范围的记载及由此解释的同等范畴内可进行多种变形及应用。

并且，在本说明书中所使用的术语(terminology)是为了适当表述本发明的优选实施例而使用的术语，其可根据使用人员、操作人员的意图或本发明所属技术领域的惯例等而不同。因此，应以本说明书整个内容为基础对本术语下定义。在整个说明书中，当某个部分“包括”某个结构要素时，除非另有特别相反的记载，否则其意指还包括其他结构要素，而并不意味着排除其他结构要素。

在一实施方式中，本发明涉及发热伴血小板减少综合征病毒，其中，在l基因的orf(6255bp)中表达的蛋白质的第1447位氨基酸为缬氨酸或第1913位氨基酸为赖氨酸，在m基因的orf(3222bp)中表达的蛋白质的第83位氨基酸为酪氨酸、第404位氨基酸为苏氨酸或第904位氨基酸为缬氨酸。本发明的一实施例中，将上述病毒命名为b-1(cb3)。

在一实例中，上述发热伴血小板减少综合征病毒属于基因型b-1，可以为在分类为以往的基因型b的病毒的l基因的orf(6255bp)中表达的蛋白质的作为第1447位氨基酸的异亮氨酸被缬氨酸取代、作为第1913位氨基酸的精氨酸被赖氨酸取代(序列13)、m基因的作为第904位氨基酸的异亮氨酸被缬氨酸取代的(序列14)病毒。

在一实例中，上述发热伴血小板减少综合征病毒可包括s基因，上述s基因包括包含序列1的碱基序列的l基因、包含序列2的碱基序列的m基因、包含序列3的碱基序列np基因以及包含序列4的碱基序列的ns基因。

在一实例中，上述发热伴血小板减少综合征病毒可包含在l基因的orf(6255bp)中表达的序列13的氨基酸序列、在m基因的orf(3222bp)中表达的序列14的氨基酸序列、在np基因的orf中表达的序列15的氨基酸序列以及在ns基因的orf中表达的序列16的氨基酸序列。

在一实施方式中，本发明涉及发热伴血小板减少综合征病毒，其中，在l基因的orf(6255bp)中表达的蛋白质的第1447位氨基酸为异亮氨酸或第1913位氨基酸为精氨酸，在m基因的orf(3222bp)中表达的蛋白质的第83位氨基酸为苯丙氨酸、第404位氨基酸为苏氨酸或第904位氨基酸为异亮氨酸。在本发明的一实施例中，将上述病毒命名为b-2(cb4)。

在一实例中，上述发热伴血小板减少综合征病毒属于基因型b-2，可以为分类为以往的基因型b的病毒的m基因的作为第83位氨基酸的酪氨酸被苯丙氨酸取代的病毒。

在一实例中，上述发热伴血小板减少综合征病毒可包括s基因，上述s基因包括包含序列5的碱基序列的l基因、包含序列6的碱基序列的m基因、包含序列7的碱基序列np基因以及包含序列8的碱基序列的ns基因。

在一实例中，上述发热伴血小板减少综合征病毒可包含在l基因的orf中表达的序列17的氨基酸序列、在m基因的orf中表达的序列18的氨基酸序列、在np基因的orf中表达的序列19的氨基酸序列以及在ns基因的orf中表达的序列20的氨基酸序列。

在一实施方式中，本发明涉及发热伴血小板减少综合征病毒，其中，在l基因的orf(6255bp)中表达的蛋白质的第1447位氨基酸为异亮氨酸或第1913位氨基酸为精氨酸，在m基因的orf(3222bp)中表达的蛋白质的第83位氨基酸为酪氨酸、第404位氨基酸为丙氨酸或第904位氨基酸为异亮氨酸。在本发明的一实施例中，将上述病毒命名为b-3(cb1)。

在一实例中，上述发热伴血小板减少综合征病毒属于基因型b-3，可以为分类为以往的基因型b的病毒的m基因的作为第404位氨基酸的苏氨酸被丙氨酸取代的病毒。

在一实例中，上述发热伴血小板减少综合征病毒可包括s基因，上述s基因包括包含序列9的碱基序列的l基因、包含序列10的碱基序列的m基因、包含序列11的碱基序列np基因以及包含序列12的碱基序列的ns基因。

在一实例中，上述发热伴血小板减少综合征病毒可包含在l基因的orf中表达的序列21的氨基酸序列、在m基因的orf中表达的序列22的氨基酸序列、在np基因的orf中表达的序列23的氨基酸序列以及在ns基因的orf中表达的序列24的氨基酸序列。

在本发明的一实施例中所分离的发热伴血小板减少综合征病毒的基因分析结果，它们的基因序列与公知的发热伴血小板减少综合征病毒的基因不同，通过对它们的系统学分类结果可知，每个基因为细化为至少三个的基因型，而并不是公知的一个b基因型组。

在本说明书中所使用的术语“取代”是指一个以上氨基酸或核苷酸分别被其他氨基酸或核苷酸代替。

本发明的发热伴血小板减少综合征病毒为负单链rna病毒，其属于布尼亚病毒科(bunyaviridae)、白蛉病毒属(phlebovirus)。并且，这种病毒为直径为80～100nm的球形病毒，以长角血蜱作为媒介，基因组由大(large(l))节段、中(medium(m))节段及小(small(s))节段组成，用于编码rna依赖性rna聚合酶(rnadependentrnapolymerase，rdrp)、糖蛋白前体(glycoproteinprecursor，m)，糖蛋白n(glycoproteinn，gn)、糖蛋白c(glycoproteinc，gc)、核衣壳蛋白(nucleocapsidprotein，np)及非结构蛋白(non-structuralprotein，nss)等六个蛋白质。负链或反义链(针对用于编码病毒蛋白的正义链或正链的反义)以反义编码蛋白质或基因，为了表达至基因的蛋白质，生成正义链或正链rna后，由此进行翻译来生成蛋白质。

在一实施方式中，本发明涉及包含发热伴血小板减少综合征病毒或其抗原作为有效成分的用于预防或治疗发热伴血小板减少综合征的免疫原性组合物。

在一实例中，本发明的免疫原性组合物可包含灭活的发热伴血小板减少综合征病毒和药剂学上可接受的载体(carrier)或佐剂(adjuvant)。

在一实例中，上述免疫原性组合物可以为疫苗组合物，其形态可选自由活疫苗、灭活疫苗、使用弱毒化发热伴血小板减少综合征病毒的基因生产的亚单位疫苗、载体疫苗、嵌合疫苗、dna疫苗及rna疫苗组成的组中。

在一实例中，上述免疫原性组合物可包含如下的发热伴血小板减少综合征病毒或它们的抗原作为有效成分：包括包含序列1的碱基序列的l基因、包含序列2的碱基序列的m基因以及包含序列3的碱基序列的s基因的发热伴血小板减少综合征病毒；包括包含序列4的碱基序列的l基因、包含序列5的碱基序列的m基因以及包含序列6的碱基序列的s基因的发热伴血小板减少综合征病毒；以及包括包含序列7的碱基序列的l基因、包含序列8的碱基序列的m基因以及包含序列9的碱基序列的s基因的发热伴血小板减少综合征病毒。

为了制备本发明的免疫原性组合物(即，疫苗)，本发明的病毒或其抗原可转化为生理学上可接受的形态。这可以基于对于流感的疫苗接种中所使用的疫苗的制备经验来实施(由stickl，h.等公开。dtsch.med.wschr.99，2386-2392)。为了制备疫苗注射剂，例如，在安瓿瓶中，优选地，在玻璃安瓿瓶中在存在1％的人白蛋白及2％的蛋白胨的条件下的100ml的磷酸缓冲盐溶液(pbs)中进行冷冻干燥。不同地，疫苗注射剂在制剂化过程中通过对病毒依次进行冷冻-干燥来生成。这种制剂可含有如甘露醇、葡聚糖、糖类、甘氨酸、乳糖或聚乙烯吡咯烷酮等附加添加剂或者如抗氧化剂或非活性气体、稳定剂或适于向生物体内给药的重组蛋白(例如，人血清白蛋白)等其他辅助剂。接着，密封玻璃安瓿瓶并可在4℃至室温下储藏几个月。但是，除非另有其他要求，否则上述安瓿瓶可优选在小于-20℃的温度下储藏。

为了预防接种或治疗，冷冻干燥物溶解于0.1ml至0.5ml的水溶液中，优选地，溶解于生理盐水或tris缓冲液中，并能够以全身或局部，即，胃肠外、皮下、肌肉内或本领域的普通技术人员公知的任意其他给药途径给药。给药形态、服用量及给药次数可由本领域的普通技术人员通过公知的方式进行优化。但是，通常，患者接受第一次疫苗接种约一个月至六周后，接受第二次疫苗接种。

在本发明中，术语“预防”是指通过给药本发明的免疫原性组合物来抑制或延缓发热伴血小板减少综合征的发生、扩散及复发的所有行为。

在本说明书中所使用的术语“治疗”是指通过给药本发明的免疫原性组合物来使发热伴血小板减少综合征及由此引起的并发症的症状得到好转或改善的所有行为。只要是本发明所属技术领域的普通技术人员，通过参照韩国大韩韩医学会等中提供的材料来确认本发明的组合物为具有效果的疾病的正确标准，可判断改善、好转及治疗程度。

在本发明中结合有效成分来使用的术语“治疗有效量”是指针对由移植排斥反应引起的疾病的预防或治疗有效的量，本发明的组合物的治疗有效可根据如给药方法、目标部位、患者的状态等各种因素而不同。因此，对人体使用时，需要一起考虑安全性及效率性决定适当量的给药量。也可以从通过动物实验决定的有效量推断对人类使用的量。例如，在决定有效量时需要考虑的事项记载于hardmanandlimbird，eds.，goodmanandgilman'sthepharmacologicalbasisoftherapeutics，10thed.(2001)，pergamonpress；以及e.w.martined.，remington'spharmaceuticalsciences，18thed.(1990)，mackpublishingco.中。

以药学有效量给药本发明的药学组合物。在本发明中所使用的术语“药学有效量”是指以可适用于医学治疗的合理的利益/风险比充分治疗疾病且不引起副作用程度的量，有效剂量水平可根据包括患者的健康状态、饮食种类、重症度、药物的活性、对药物的敏感度、给药方法、给药时间、给药途径及排泄比例、疗程、配合或同时使用的药物在内的因素及其他医学领域中公知的因素来决定。本发明的组合物可作为单独的治疗剂给药或者可与其他治疗剂联用来给药，可与以往的治疗剂依次或者同时给药，可进行单次给药或多次给药。考虑到上述全部因素，优选地，给药在没有副作用的情况下可得到最大效果的量，这可由本领域的普通技术人员容易决定。

本发明的药学组合物可包含通常使用于生物学制剂中的载体、稀释剂、赋形剂或它们中两种以上的组合。在本发明中所使用的术语“药学上可接受”是指对于暴露在上述组合物中的细胞或人类没有毒性的特性。上述载体只要是将组合物适于传递至生物体内的载就不受特别限制，例如，可将在merckindex，13thed.，merck&co.inc.中记载的化合物、生理盐水、无菌水、林格氏液、缓冲生理盐水、葡萄糖溶液、麦芽糊精溶液、丙三醇、乙醇及这些成分中的一种成分以上混合来利用，根据需要，可添加抗氧化剂、缓冲液、细胞抑制剂等其他常规的添加剂。并且，进一步添加稀释剂、分散剂、表面活性剂、结合剂及润滑剂来制剂化为如水溶液、悬浊液、乳浊液等注入用剂型、丸剂、胶囊、颗粒或片剂。进一步地，可利用本领域中适当的方法或在雷明顿药物科学(remington'spharmaceuticalscience，mackpublishingcompany，eastonpa，18th，1990)中记载的方法并根据各种疾病或成分来进行优选的制剂化。

在一实例中，上述药学组合物可以为选自由口服剂型、外用剂、栓剂、灭菌注射用液及喷雾剂组成的组中的一种以上剂型，口服剂型或注射剂型更优选。

在本发明中所使用的术语“给药”是指以任意适当的方法向对象或患者提供规定的物质，可按照期望的方法进行胃肠外给药(例如，适用静脉内、皮下、腹腔内或局部注射剂型)或者口服给药，给药量的范围根据患者的体重、年龄、性别、健康状态、饮食、给药时间、给药方法、排泄率及疾病的重症度等而不同。用于本发明的组合物的口服给药的液态制剂为悬浊剂、内用液剂、乳剂、糖浆剂等，除了作为通常所使用的简单稀释剂的水、液体石蜡之外，还可包含如湿润剂、甜味剂、芳香剂、保存剂等多种赋形剂。用于胃肠外给药的制剂包括灭菌的水溶液、非水性溶剂、悬浊剂、乳剂、冷冻干燥制剂、栓剂等。还可以通过能够将活性物质移动至靶细胞的任意装置给药本发明的药学组合物。优选的给药方式及制剂为静脉注射剂、皮下注射剂、皮内注射剂、肌肉注射剂、点滴注射剂等。可通过生理盐溶液、林格氏液等水性溶剂、植物油、高级脂肪酸乙酯(例如，油酸乙酯等)、醇类(例如，乙醇、苯甲醇、丙二醇、丙三醇等)等非水性溶剂等来制备注射剂，可包含用于防止变质的稳定剂(例如，抗坏血酸、亚硫酸氢钠、焦亚硫酸钠、bha、生育酚、edta等)、乳化剂、用于调节ph的缓冲剂、用于抑制微生物繁殖的保存剂(例如，硝酸苯汞、硫柳汞、苯扎氯铵、苯酚、甲酚、苄醇等)等药学载体。

在本发明中所使用的术语“对象”是指上述癌症发病或者会发病的包括人类在内的猴、牛、马、羊、猪、鸡、火鸡、鹌鹑、猫、狗、小鼠、大鼠、兔或豚鼠等所有动物，“样品”可以为从这些动物中分离的全血、血浆、血清、尿或唾液。

本发明的药学组合物还可以包含药剂学上可接受的添加剂，此时，作为药剂学上可接受的添加剂可使用淀粉、糊化淀粉、微晶纤维素、乳糖、聚维酮、胶态二氧化硅、磷酸氢钙、乳糖、甘露醇、饴糖、阿拉伯树胶、预胶化淀粉、玉米淀粉、粉状纤维素、羟丙基纤维素、欧巴代、羧甲淀粉钠、巴西棕榈蜡、合成硅酸铝、硬脂酸、硬脂酸镁、硬脂酸铝、硬脂酸钙、白糖、葡萄糖、山梨糖醇及滑石粉等。优选地，相对于上述组合物，可包含0.1重量份至90重量份的本发明药剂学上可接受的添加剂，但并不限定于此。

在一实施方式中，本发明涉及使用本发明的病毒或其抗原进行免疫应答来生产的抗体。

上述抗体不仅是全(whole)抗体形态，而且还包含抗体分子的功能性片段。全抗体为具有两个全长的轻链(lightchain)及两个全长的重链(heavychain)的结构，各个轻链通过二硫键(disulfidebond)与重链连接。抗体分子的功能性片段是指具有抗原结合功能的片段，抗体片段例如包括：(i)由轻链的可变区(vl)及重链的可变区(vh)和轻链的恒定区(cl)及重链的第一个恒定区(ch1)组成的fab片段；(ii)由vh结构域及ch1结构域组成的fd片段；(iii)由单抗体的vl结构域及vh结构域组成的fv片段；(iv)由vh结构域组成的dab片段(wardesetal.，nature341:544-546(1989)]；(v)分离的cdr区；(vi)包含两个连接的fab片段的作为二价片段的f(ab')2片段；(vii)通过以使vh结构域及vl结构域形成抗原结合部位的方式结合的肽衔接物结合的单链fv分子(scfv)；(viii)双特异性单链fv二聚物(pct/us92/09965)；以及(ix)通过基因融合制备的作为多价或多特异性片段的双体(diabody)wo94/13804)等。

在一实施方式中，本发明涉及包含发热伴血小板减少综合征病毒或其抗原或针对其的抗体的发热伴血小板减少综合征病毒的诊断试剂盒。

在一实例中，上述试剂盒可包括包含本发明的病毒的病毒试样及上述抗原-抗体复合物检测用试剂。上述抗原-抗体复合物检测用试剂包括放射免疫分析、酶联免疫吸附测定(enzymelinkedimmunosorbentassay，elisa)或免疫荧光分析用试剂。

在一实例中，可利用可简单地通过抗原抗体结合检测到的奥克特洛尼(ouchterlony)平板、免疫印迹、如crossedie、rocketie、fusedrocketie、affinityie等免疫电泳(immunoelectrophoresis)来实现抗原-抗体复合物的检测。在这种方法中所使用的试剂或物质为公知的，例如，可通过抗原-抗体之间反应、和与抗原特异性结合的底物、核酸或肽适体、与复合物相互作用的受体或配体或辅因子之前的反应来检测，或者可利用质量分析器。可将上述与本发明的抗原-抗体复合物特异性相互作用或结合的试剂或物质与核方式或纳米粒子(nanoparticle)一起使用。上述免疫分析或免疫染色的方法记载于enzymeimmunoassay，e.t.maggio，ed.，crcpress，bocaraton，florida，1980；gaastra，w.,enzyme-linkedimmunosorbentassay(elisa)，inmethodsinmolecularbiology，vol.1，walker，j.m.ed.,humanapress，nj，1984中。可通过分析上述的免疫分析过程的最终信号的强度，即，进行与正常试样之间的信号对照来诊断是否感染。

在一实施方式中，本发明涉及包含发热伴血小板减少综合征病毒或其抗原或针对其的抗体的诊断组合物。

优选地，以能够被检测的方式标记诊断组合物中所使用的本发明的上述化合物。在标记生物分子时可使用的多种方法为本领域的普通技术人员公知的，在本发明的范畴内考虑。上述多种方法记载于tijssen，'practiceandtheoryofenzymeimmunoassays'，burden,rhandvonknippenburg(eds)，volume15(1985)，'basicmethodsinmolecularbiology'；davislg，dibmermd；batteyelsevier(1990)，mayeretal.，(eds)'immunochemicalmethodsincellandmolecularbiology'academicpress，london(1987)，orintheseries'methodsinenzymology'，academicpress，inc中。

本领域的普通技术人员公知的有标记方法和多种其他标记。可在本发明中使用的标记种类例如有酶、放射性同位素、胶体态金属、荧光化合物、化学发光化合物及生物发光化合物。

通常所使用的标记为荧光物质包括(例如，荧光黄、罗丹明、德克萨斯红等)、酶(例如，辣根过氧化物酶、β-半乳糖苷酶、碱性磷酸酶)、放射性同位素(例如，³²p或²⁵i)、生物素、洋地黄毒苷、胶体态金属、化学发光或生物发光化合物(例如，二氧杂环丁烷、鲁米诺或吖啶)。酶或生物素基的共价键合法、碘化法、磷酸化法、生物素法等标记方法为在本领域中公知的。

检测方法为射线自显迹法、荧光显微镜、直接及间接酶反应等，但并不限定于此。通常所使用的检测分析法为放射性同位素或非放射性同位素方法。其中，通常使用免疫印迹、叠加分析法、放射免疫分析(radioimmunoassay，ria)及免疫放射分析(immuneradioimmunometricassay，irma)、酶联免疫分析(enzymeimmunoassay，fia)、酶联免疫吸附分析(enzymelinkedimmunosorbentassay，elisa)、荧光免疫分析(fluorescentimmunoassay，fia)以及化学发光免疫分析(chemioluminescentimmuneassay，clia)。

在一实施方式中，本发明涉及发热伴血小板减少综合征病毒抗体的检测方法，包括：在可形成抗原-抗体复合物的条件下，使从样品中分离的试样与本发明的病毒或其抗原接触的步骤；以及检测抗原-抗体复合物的形成的步骤。

在一实施方式中，本发明涉及在非人类动物中生产针对发热伴血小板减少综合征病毒的抗血清的方法，包括：向非人类动物给药适于诱导免疫应答的有效量的本发明的病毒或其抗原的步骤；以及收集含有针对上述发热伴血小板减少综合征病毒的抗体的抗血清或血浆的步骤。

在一实施方式中，本发明涉及与发热伴血小板减少综合征的诊断有关的信息的提供方法，包括：通过使从样品中分离的试样与本发明的病毒或其抗原接触来形成抗原-抗体复合物的步骤；以及检测上述抗原-抗体复合物的形成的步骤。

具体实施方式

通过以下实施例更详细地说明本发明。但是，以下实施例仅用于使本发明的内容具体化，而本发明并不限定于此。

实施例1.分离病毒

利用大学附属医院就诊患者中疑似发热伴血小板减少综合征的患者的血液、动物(山羊及遗弃犬)的血液及野生蜱均质物并通过实时荧光定量pcr(realtimepcr)、pcr及elisa分析确认发热伴血小板减少综合征病毒的阳性/阴性。具体地，感染病毒前天，将veroe6细胞分株于12孔板中后，以大于60％的细胞密度进行培养后，用pbs清洗细胞，将300μl的疑似感染患者的血清(以3000rpm离心分离整个血液20分钟来获得的血清)处理1小时来感染。感染后，去除血清并用pbs清洗后，用含1％的fbs的dmem培养基更换并培养2周。2周后，通过rt-pcr(逆转录(reversetranscription)后用实时荧光定量pcr确认)及免疫荧光分析法(immunofluoresenceassay)(在实验室，将小鼠(mouse)发热伴血小板减少综合征病毒np抗体用作第一抗体以及将共轭fitc的抗体用作第二抗体)确认病毒分离与否，在没有分离病毒的情况下，通过将第一次感染过的上清液感染在另一个veroe6细胞的方式分离病毒。将分离的病毒命名为cb3/2016、cb7/2017及cb6/2016。

实施例2.分析分离的病毒的基因

分别对利用veroe6细胞分离的病毒cb3/2016、cb7/2017及cb6/2016进行逆转录(reverse-transcription)、pcr及下一代测序(nextgenerationsequencing，ngs)来确认各病毒的l、m、s(np、ns)基因的全长序列。具体地，从各病毒中提取rna并通过逆转录(reverse-transcription)pcr来制备cdna。之后，对各sfts病毒的l、m、s基因进行pcr来获得各自的全长基因。使用ngs方法进行基因序列分析，使用illuminanexteraxtkit并根据由因美纳(illumina)公司提供的协议对各病毒的l、m、s基因进行标签化(tagmentation)及索引pcr(indexpcr)。之后，将最终样品利用illiminaminiseq测序系统生成fastaq文件，利用clcmainworkbenchprogram所生成的文件的基因序列分析各自的全长基因序列。将确认到的基因序列与在韩国、中国及日本中分离的以往的病毒的基因整合来进行基因分析，通过其结果确认，在本发明中分离的发热伴血小板减少综合征病毒cb1、cb3及cb4为与目前在中国或韩国分离的病毒(yuxjetal.,n.engl.j.med.2011)及在韩国首次分离的(gangwon/2012)病毒的基因具有遗传性区别的新型基因型(genotype)的发热伴血小板减少综合征病毒。同时，使用mega7.0程序对本发明的病毒与在韩国、中国、日本分离的病毒的l、m、s(np、ns)基因进行系统学基因分析，通过其结果确认，本发明的病毒接近于韩国分离株所属最多的b组，但是，基因l、m及s(np及ns)基因中显示与以往的病毒不同之处，细化为至少三个以上组来区分(图1至图4)。对此，将在本发明分离的三种基因型的发热伴血小板减少综合征病毒细化为b-1(cb3/2016)、b-2(cb7/2017)及b-3(cb6/2016)来进行命名。

实施例3.确认各病毒的l、m及s基因氨基酸序列差异

3-1.l基因氨基酸序列差异

以在本发明分离的新型细化基因型b-1、b-2及b-3的病毒的l、m及s基因的可读框(openreadingframe，orf)为基准分析氨基酸序列，其结果，l及m基因具有差异。具体地，b基因型中l基因的orf(6255bp)编码rdrp，本发明的基因型b-1病毒中，rdrp的第1447位氨基酸为缬氨酸或第1913位氨基酸为赖氨酸，基因型b-2病毒及b-3病毒中，rdrp的第1447位氨基酸为异亮氨酸或第1913位氨基酸为精氨酸(图5的a部分及表1)。

表1

3-2.m基因氨基酸序列差异

分析在本发明分离的新型细化基因型b-1、b-2及b-3的病毒的l、m及s基因的氨基酸序列，其结果，l及m基因具有差异。具体地，b基因型中m基因的orf(3222bp)编码gn及gc蛋白，本发明的基因型b-1病毒中，m基因的orf的第83位氨基酸为酪氨酸、第404位氨基酸为苏氨酸或第904位氨基酸为缬氨酸，基因型b-2病毒中，m基因的orf的第83位氨基酸为苯丙氨酸、第404位氨基酸为苏氨酸或第904位氨基酸为异亮氨酸，基因型b-3病毒中，m基因的orf的第83位氨基酸为酪氨酸、第404位氨基酸为丙氨酸或第904位氨基酸为异亮氨酸(图5的b部分及表1)。

实施例4.比较各基因型的基因的同源性

针对包括本发明的细化基因型b-1、b-2及b-3在内的目前在韩国分离的发热伴血小板减少综合征病毒进行基因分析，比较分析了各基因型的l、m及s基因同源性。通过其结果确认，在属于相同基因型的病毒之间的基因同源性(nucleotidelevel)为约96～100％，与此相反，不同基因型病毒之间的基因同源性较低，为91～97％(表2至表5)。并且，在本发明的基因型b-3组的情况下，相同组中的同源性较低，为约95～100％左右，在b-3组中也可以细化为其他基因型组。

表2

比较l基因的同源性

表3

比较m基因的同源性

表4

比较s(np)基因的同源性

表5

比较s(ns)基因的同源性

实施例5.分析各基因型的交叉免疫原性及确认疫苗效果

5-1.各基因型的交叉免疫原性分析

通过frnt50(fiftypercentoffocusreductionneutralizationtest)分析各基因型的交叉免疫原性。具体地，使在本发明分离的新型细化基因型b-1、b-2及b-3的病毒大量增殖后，分别添加福尔马林(0.05％)进行灭活后，通过三次的病毒分离(isolation)确认灭活与否。采用20％的蔗糖(sucrose)灭活的上述疫苗(inactivatedwholevaccine)进行超离心分离来生产蛋白质后，将其对雪貂进行免疫化。2周后，进一步对雪貂进行免疫化后(两次免疫间隔之间为两周)，采血并分离血清。在56℃的温度下灭活所分离的血清30分钟后，以1/10比例稀释后，进行每次2倍的阶段性稀释。在37℃的温度下，将以200ffu/ml稀释的病毒与阶段性稀释的病毒以1：1的比例进行反应。清洗分株于6孔板中的veroe6细胞后，用经上述反应的病毒进行感染，1小时后进行清洗后，将含1％的fbs的0.8％的dmem琼脂糖凝胶倒入细胞中。感染5天后，采用福尔马林固定3小时后，清洗三次后，在室温下各进行5分钟的10％的tritonx-100处理。之后，清洗三次并用5％的bsa进行封闭。使用所生产的多克隆np抗体(polyclonalnpantibody)作为第一抗体来进行培养后清洗三次，用第二抗体使hrp共轭(hrpconjugated)抗体反应1小时并清洗后，用dab发色来确认结果。应解释为，比仅感染病毒的孔的病灶形成(foucusforming)降低至50％的值为有效。

表6

确认b-1、b-2及b-3的交叉免疫原性的结果如上述表6所示，针对各病毒显示出最高的血清抵抗。因此，在属于相同的基因型的病毒之间显示出高交叉中和反应性，但在各自不同的基因型之间的病毒之间显示出较低的中和反应性。

5-2.确认疫苗效果

使在本发明分离的新型细化基因型b-1、b-2及b-3的病毒大量增殖后，分别添加福尔马林(0.05％)进行灭活后，通过三次的病毒分离(isolation)确认灭活与否。对各病毒将灭活的上述疫苗(inactivatedwholevaccine)以两周为间隔对每五只雪貂进行免疫化两次后，以1×10^7.6/ml对各病毒进行挑战接种。

通过挑战接种结果确认，对照组动物在10天内都在被挑战病毒感染时死亡，但接种疫苗的组的所有雪貂存活，并在感染2～8天后确认体温上升、体重下降，之后开始恢复(图6)。

概括以上的结果，在发热伴血小板减少综合征病毒中存在具有多种基因的多种基因型的病毒，在属于相同基因型的病毒之间显示出较高基因同源性及低交叉免疫原性，与其他基因型的病毒之间显示出较低基因同源性及低交叉免疫原性，因此，为了产生针对多种基因型的交叉免疫原性，可推断为某个特定基因型的病毒的防御能力是受限的。

因此，可将属于本发明的细化基因型b-1、b-2及b-3的新型病毒cb3/2016、cb7/2017及cb6/2016有用地利用为对基因型b的发热伴血小板减少综合征病毒具有优秀的交叉免疫原性的疫苗。

<110>鉴定生物株式会社

<120>新型发热伴血小板减少综合征病毒

<130>po1903-006

<160>24

<170>kopatentin3.0

<210>1

<211>6255

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1l基因

<400>1

atggacttggaagtgctttgtggtaggataaacgtggagaatgggctgtctcttggagaa60

ccaggcctgtacgaccaaatctacgacaggccagggctgccagacctagatgtgactgtc120

gatgccacaggtgtgacagtggacataggggctgtgccagactcagcatcacaactgggc180

tcatcaatcaatgctgggttgatcacaatccagctctctgaagcatataagatcaatcat240

gacttcacattttctggcctgtcaaagacaacagaccgacgcctctcagaggtattcccc300

attacccatgatggttctgatgggatgacccctgatgtgatccacaccagattggatgga360

actattgtggtggttgaattttcaaccactaggagccataacattgggggcctggaggca420

gcatataggacaaagatagaaaaatatagggacccaatctcaaggcgtgttgatatcatg480

gagaacccgagagtcttctttggtgttattgtagtctcgtcaggaggggttctgtccaac540

atgcccctgactcaggatgaggcagaggagctcatgtacaggttctgcatagccaatgaa600

atctacactaaggctagatctatggatgcagacattgagctacagaagagtgaagaagag660

cttgaggctatcagcagggcactttcattcttcagtttgttcgagcctaacattgaaaga720

gtggaaggaacattccccaattcagaaattgagatgctggaacagtttctctcaacacca780

gctgatgttgacttcatcaccaagaccctcaaagcaaaagaggtggaggcctatgctgat840

ctttgtgacagccactacctaaagcctgaaaaaaccattcaggagcgtctagagatcaat900

agatgtgaggctattgacaaaactcaggacctcctagctggcctacatgcaaggagcaac960

aagcaaacatcattgaatcgagggacagtcaaactcccgccctggctgccaaagccatca1020

agtgagtcaatagacatcaagaccgactcaggctttggttccttaatggatcatggcgca1080

tatggtgagctatgggcaaagtgccttctagatgtctcgttaggcaatgtggagggggta1140

gtcagtgaccctgcaaaagagcttgacattgctatctctgatgacccagaaaaagacacc1200

cccaaagaggcaaagataacctataggcgattcaagcctgccttaagttcaagtgcccgg1260

caagaattttctctccaaggagtggaggggaagaagtggaagagaatggcagcaaaccag1320

aagaaagagaaggagtcccatgagacattgagccctttcttggatgttgaagacattggg1380

gatttcctgacattcaacaatcttcttgcagattcgaggtatggagatgagtccatccag1440

agagctgtgtcaatcttgttggaaaaggcatctgccatgcaggacacagagctcactcat1500

gctctcaacgactcattcaagaggaacctaagcagcaatgtggttcagtggtccctttgg1560

gtttcctgtttggcgcaggagctagctagtgctctgaagcagcactgcagggctggtgag1620

ttcatcatcaagaagctgaagttctggcctatctatgtcattatcaagccgaccaaatcg1680

tcatctcacatcttctacagcttagggatccgcaaggctgacgtgacaaggaggctcact1740

ggtagagtcttctctgacaccattgatgcaggggaatgggagctaacagagttcaaaagc1800

ctgaagacatgtaagctcacgaaccttgtcaacttaccatgcaccatgctgaactcaata1860

gctttctggagagagaagctgggcgtggctccatggctggtccggaagccttgttcagag1920

ctcagggagcaggtgggcctgaccttcctgatcagtctggaggacaagtctaagactgag1980

gagatcatcaccttgacaaggtacacccagatggagggctttgtctctcctcccatgctg2040

cccaaaccccaaaagatgctagggaaactggatggacctttgagaaccaagctacaggtt2100

tacctcctcaggaagcatctggattgcatggtgcgaattgcttctcagccattcagccta2160

atcccaagagaggggagagtagaatggggaggaacattccatgccatctcaggccggtcc2220

acaaatcttgagaatatggtgaacagctggtacattgggtactacaagaacaaagaggag2280

tcaacagaactaaatgctctcggagaaatgtataagaagattgtggagatggaagaggac2340

aagcccagcagccctgagtttctggggtggggggacactgattcccctaagaagcatgaa2400

ttctcacggagcttcctgagagctgcttgctcatctctggaaagagaaattgctcagcga2460

catggaagacaatggaagcagaaccttgaggagcgagtcctgagagagattgggaccaag2520

aacatcctggaccttgcatccatgaaggctactagcaacttttccaaagactgggagctc2580

tactcagaagtccagaccaaagagtaccataggtccaaactgctggagaagatggccaca2640

ttgattgagaagggggttatgtggtacattgatgctgtgggtcaggcatggaaggcagtt2700

ctggatgacgggtgcatgcgaatctgtctcttcaaaaagaatcagcatggtggcctcaga2760

gagatctacgttatggatgcaaatgcccggctcgtgcagtttggggttgagaccatggct2820

aggtgtgtctgtgagctgagcccacatgagactgttgccaaccctaggctcaagaattcc2880

atcatagagaaccatgggctgaagtcagcccgtagtcttggccctggctctataaacata2940

aactcatccaatgatgccaggaagtggaatcaggggcactacacaacaaagctagctcta3000

gttctttgttggttcatgccagccaaattccacagattcatttgggctgccatttccatg3060

tttcggagaaaaaagatgatggtggacctaaggtttttagctcacctcagttctaaatct3120

gagtctaggtcatctgatccatttagggaagcaatgacagacgccttccatggcaatagg3180

gaagtctcatggatggacaaagggcgaacttacataaagacagagacaggtatgatgcag3240

ggtatactgcactttacatccagcctcctccactcttgtgttcagagcttttacaagtct3300

tatttcgtctcgaaactcaaggagggctacatgggagaaagcatcaatggggtggtggat3360

gtcatagaaggctctgacgactcagcgatcatgatcagcatacgccccaagtcagacatg3420

gatgaagtccgatcaaggttctttgttgctaacttactccactctgtaaagttcttgaac3480

cctttgtttgggatctactcatcagagaaatcaacagtgaacacagtgtattgtgtcgag3540

tataactctgaattccatttccacaggcacttggttcgacccacactgagatggatagca3600

gcatctcaccaaatctcagagactgaggcccttgcaagcaggcaagaggattattcaaac3660

cttctaacccagtgcttggagggaggggcctcattctctcttacctacctcatacagtgc3720

gctcagctcctgcaccactacatgcttctaggactatgcttacaccccttgtttggaact3780

ttcatggggatgctgatatcagacccagatccagccctagggttcttcctcatggacaac3840

cctgcattcgcaggaggagcaggatttagattcaatctgtggagagcctgcaagactaca3900

gaccttgggcggaagtatgcatattattttaatgagatacagggtaaaacaaagggagat3960

gaggactacagagctctggacgccacatcaggaggaaccctcagccactctgttatggtg4020

tactggggggacaggaagaagtatcaggccttattgaacaggatgggccttcctgaggac4080

tgggtggagcagatagatgagaatcccggtgtcctttacaggagagctgccaacaagaag4140

gaactactcttaaaactggcagagaaggttcattcacctggtgtgactagcagcctgagt4200

aaagggcatgtagtgcctcgggtggtggcagcaggagtataccttctctcacgccattgc4260

tttcgctttagctcaagcatccatggaaggggctcagcacagaaggctagccttataaaa4320

ctgttgatgatgtcttctgtttctgccatgaagcatgggggctcactaaaccctaatcag4380

gagcgaatgctcttccctcaggctcaagagtatgacagagtatgcacattgcttgaggag4440

gttgaacacctaacagggaaatttgttgttagggagaggaacattgtcaggagccgcata4500

gacttgttccaagagccagtggacttgcggtgcaaggcagaagatctggtgtcagaggtg4560

tggtttggcctgaaaaggactaaacttggaccccgtctcctcaaggaagagtgggacaaa4620

cttagggcctcatttgcatggctgagcacagacccatctgaaacattgagggatggtcct4680

tttcttagccatgtgcagtttaggaacttcatagcccacgttgatgccaaatcaagatca4740

gtcaggctcctaggtgcccccgtaaagaagtcaggtggggtcaccactataagccaagta4800

gtcagaatgaacttcttcccaggttttagcctagaagctgagaagagcttagacaatcag4860

gaaagacttgagagcatctccatcctcaagcatgtcttgttcatggtcttgaatggccca4920

tacactgaggagtacaagctggaaatgatcatagaggccttctctactcttgtgatacct4980

cagccatcagaggtcatcaggaaatcaaggaccatgactttgtgcctcttatcaaattac5040

ttgtctagtaggggtgggtccattctagaccagattgagagggcacagtcaggcactcta5100

gggggcttcagcaagccccagaagactttcattaggccaggaggtggtatcggctacaag5160

ggaaagggtgtgtggactggagtgatggaggacacccatgttcaaattctgatagatgga5220

gatgggactagtaactggcttgaggagatcaggctcagtagtgatgccagactttatgat5280

gtcattgagtccatccgaaggttatgtgatgacctcgggatcaacaacagggtggcatct5340

gcatatagaggccattgcatggttaggctgagtggattcaagatcaagccagcatcaagg5400

actgatggctgcccagtcaggattatggaaaggggcttcaggatcagggaactccaaaac5460

ccagatgaggtcaagatgagagtgagaggtgacatcctcaacctctctgtcaccattcaa5520

gaaggaagggtcatgaacattctaagctacaggccgagagacactgatatatcagaatca5580

gccgcagcatatctctggagcaatcgagacctcttctcctttgggaagaaggagccatcc5640

tgcagctggatctgcttgaaaactcttgacaattgggcctggtcacatgcctcagttctc5700

ctggcaaatgataggaagacccaaggcattgataataaggctatggggaacattttcagg5760

gactgtctcgagggttctcttagaaagcaagggctgatgaggtcaaagctcacagagatg5820

gtggagaagaatgtagttcctttaacaactcaagagcttgtcgacatcctggaggaggac5880

atagacttttcagatgtcatagctgtggaactctcagagggatcacttgatattgaatcc5940

atctttgatggggcacctatcttgtggtctgctgaggtggaagagtttggggaaggagtg6000

gtggctgtgagctattccagtaagtactatcatctaaccctgatggaccaagctgccatc6060

acaatgtgtgcgatcatgggtaaggaaggctgtagagggctccttactgagaagagatgc6120

atggcagccatacgagagcaggtacggccattcctcatattcctgcaaatccctgaggat6180

agcatttcttgggtgtctgatcagttctgcgactccaggggtcttgatgaggagagcacc6240

attatgtggggttga6255

<210>2

<211>3222

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1m基因

<400>2

atgatgaaagtcatctggttctcctctctgatctgcttagtcattcaatgcagtggggat60

acgggtccaatcatatgcgcagggcccatccactcaaacaagagtgccaacataccccac120

ctgcttggctactctgagaagatttgtcagatagatcggctgatacatgtttcgtcatgg180

ctgagaaaccattcacaatttcagggctatgtggggcagcgaggtggacgctctcaggta240

agctattacccagctgaaaactcttactcaaggtggagtggacttctaagcccctgtgat300

gcagattggcttgggatgcttgttgtgaagaaggccaaagggtctgatatgatagttcct360

gggccttcatacaagggaaaagtcttttttgaacggccaacttttgatggatacgtaggc420

tggggctgtggcagtgggaagtcaaggacagagtcaggggagctctgcagctcagactca480

gggaccagttccggtcttctgccctcagatagggttctctggataggtgatgttgcttgc540

cagcctatgacacccatccctgaggagacatttctggagctgaagagttttagccagagt600

gaattcccagacatatgcaaaattgatggcattgtgttcaaccagtgtgagagtgagagt660

ctacctcaacccttagatgttgcgtggatggatgtaggccactctcataaaatcatcatg720

agggagcacaagactaaatgggtacaagagagctcatctaaggattttgtgtgctacaag780

gaagggactgggccatgttctgaatcagaagaaaagacttgcaagaccagtggatcatgt840

aggggggacatgcagttttgcaaggtggcaggttgtgaacatggggaagaagcatctgat900

gccaagtgtagatgctcactagtgcacaagcccggggaagttgttgtgtcatatggaggg960

atgcgtgtcagaccaaagtgttatggcttctccagaatgatggcaacactagaggtgaac1020

ccaccagagcaaaggattggccaatgcactggctgccatcttgaatgcataaatgggggt1080

gtgaggctaatcactctgactagtgagctcaagtcagctactgtctgtgcttcccacttt1140

tgtagttctgccacaagtggcaagaaaagcacggagattcaatttcactcagggtcatta1200

gttgggaaaacaacaatacacgtcaaaggggctttggtggatggaactgaattcacattt1260

gagggcagttgcatgttcccagatggttgtgacgcagtggactgcacattctgtcgtgag1320

tttctaaaaaatcctcagtgctaccctgcaaagaaatggctgttcatcattattgtcatc1380

ctccttggatatgcaggcctcatgctacttaccaatgtccttaaggcaatcggggtttgg1440

ggatcatgggtcatagctccagtgaagctaatgtttgccatcataaagaaactgatgaga1500

tctgtgagctgcttgatggggaaattaatggataggggaaggcaagtgatccatgaagaa1560

ataggggagaatagagagggcaaccaagatgatgttaggatcgagatggcaagacccaga1620

agggtaaggcattggatgtactcacctgtcatcctgactattctagcaattgggcttgcc1680

gagggctgcgatgagatggtccatgctgactccaaacttgtttcgtgcaagcaagggagc1740

ggaaacatgaaggaatgtgtcacaactgggagggcactccttcctgcggtgaacccagga1800

caagaggcatgtctgcacttcacggcacctgggagtccggactcaaaatgtctcaaaatt1860

aaggtcaagaggattaacctaaaatgtaagaagtcatcatcatattttgttcctgatgct1920

cggtctaggtgtacatctgtgaggagatgtcgctgggcaggagactgccagtctgggtgc1980

ccctctcatttcacgtccaactccttctctgatgattgggcaggtaaaatggacagggca2040

ggtctaggattcagtgggtgctctgatggatgtggaggagcagcctgcggctgctttaat2100

gcagccccttcatgcatcttttggaggaaatgggtagagaacccgcatgggatcatctgg2160

aaagtatctccatgtgctgcatgggtcccatcagcagtcatagagctaacaatgccctca2220

ggggaagtgaggacattccaccccatgagcggcatccctacacaagtcttcaagggtgtg2280

agtgtgacgtacttaggctcagatatggaggtgtctggcttgactgacctgtgtgagata2340

gaagagctcaagtccaagaagctggcattagccccctgcaatcaggctggcatgggggtt2400

gtaggcaaggttggagagatacagtgcagtagcgaggaaagtgcccgtaccataaaaaaa2460

gatgggtgtatatggaatgctgaccttgtgggcatagagctacgagtggatgacgctgtg2520

tgctactctaagatcactagtgtggaggcagttgcaaactactctgccatacccaccact2580

attggggggctgaggtttgagagaagccatgacagccagggtaaaatatctggtagcccc2640

ttagatataacagccataagagggtctttttcagttaattatagaggccttcgactgagc2700

ctctcagaggttactgctacttgcacaggagaggtgacgaatgtgagtgggtgttactct2760

tgcatgacaggcgccaaagtctccatcaaactgcatagcagcaaaaatagcactgcccat2820

gtaagatgcaaaggggatgagacagcattcagtgtcctagtgggagttcatagctatact2880

gtcagtctcagctttgaccatgcagtggtcgatgagcagtgccagctgaactgtggaggg2940

catgagagtcaagtgactctaaaaggcaacctcatcttcctggatgtcccaaaatttgtg3000

gatggcagctatatgcagacatatcatagttctgtgcccacaggggccaacatcccaagc3060

cctacagactggctgaatgccttgttcggcaatgggctgagtaggtggattctgggggta3120

ataggggttctactggggggattggctctctttttcttaatcatgtctctgttcaaattg3180

ggaacaaaacaggtatttcgatcaaggacgaagctggcttaa3222

<210>3

<211>738

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1np基因

<400>3

atgtcagagtggtccaggattgcagtggagtttggtgagcagcagctcaatttgacggag60

cttgaggacttcgcgagagagctggcctatgaaggccttgatcctgctttgatcatcaag120

aagctgaaggagacaggtggggatgattgggtgagggatacaaagttcatcattgtcttt180

gccctgactcgaggcaataagattgtcaaggcatcagggaaaatgtcaaactcagggtct240

aagaggttgatggcactccaagagaagtatggactggttgagagggcagaaaccaggctc300

tcaatcactcctgtgagggtggcacagagcctacccacttggacatgtgctgctgcagca360

gccttaaaggagtatcttccagttgggccagctgtcatgaacctgaaggtcgagaattat420

cctccagagatgatgtgcatggcctttgggtccctgattccaactgcgggggtgtcagaa480

gctacaacgaagaccctgatggaggcctactctctgtggcaagatgccttcacaaagact540

atcaatgtgaagatgcgcggagccagtaagacagaggtttacaactccttcagggatcct600

ctccatgctgctgtgaacttcgtctttttcccccaagatgttcgggtgaagtggctgaag660

gccaagggaatccttggcccagatggggtccccagcagagctgctgaggttgctgctgct720

gcttacagaaacctgtaa738

<210>4

<211>882

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1ns基因

<400>4

atgtcgctgagcaaatgctccaacgttgacctcaaatctgtggcaatgaatgccaacact60

gtcaggcttgagccatctctaggagagtaccccactcttaggagagacctcgttgaatgc120

tcttgtagtgtgttgactctatcaatggttaagaggatgggcaagatgaccaacacagta180

tggttgtttggtaacccaaaaaatcctcttcaccagcttgagcctggactcgagcagctg240

ttggacatgtactacaaggacatgaggtgctactcccagagagagctgagtgctcttagg300

tggcctagtgggaagccatctgtatggttcctgcaggcagctcatatgttcttctccatc360

aagaacagctgggcaatggaaaccggcagagagaattggcggggcctcttccacaggata420

acaaaaggcaaaaagtatctttttgaaggagacatgatattggattctcttgaggccata480

gagaagcgaaggcttagacttgggctacctgagatcctaataactggactatccccgatt540

ctggatgtggccctcctccagatagagtcacttgcaaggctaagaggcatgagcttgaac600

caccacttattcacatcttcctcattgcgtaagcctttgttggactgttgggacttcttt660

attcctatccggaaaaagaggacagatggctcatacagtatcttggatgaggatgatgaa720

cttggggtccttcaaggttacccatatctgatggcacactatttgaataggtgcccattc780

cacaacctcatcaggtttgatgaagagctgagaactgcagccctaaacaccatctgggga840

agagattggccggccattggtgacctcccgaaggaggtctaa882

<210>5

<211>6255

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2l基因

<400>5

atgaacttggaagtgctttgtggtaggataaacgtggagaatgggctgtctcttggagaa60

ccaggcctgtacgaccaaatctacgacaggccagggctgccagacctagatgtgactgtc120

gatgccacaggtgtgacagtggacataggggctgtaccagactcagcatcacaactgggc180

tcatcaatcaatgctgggttgatcacaatccagctctctgaagcatataagatcaatcat240

gacttcacattttctggcctgtcaaagacaacagaccgacgcctctcagaggtattcccc300

attacccatgatggttcagatgggatgacccctgatgtgattcataccagattggatgga360

accattgtggtggttgaattttcaaccactaggagccataacattgggggcctggagacg420

gcatatagaacaaagatagagaaatatagggacccaatctcaaggcgtgttgatatcatg480

gagaacccgagggtcttctttggcgttattgtagtctcgtcaggaggggttctatccaac540

atgcccctgactcaggatgaggcagaggagctcatgtacaggttctgcatagccaatgag600

atctacaccaaggctagatctatggatgcagacattgagctacagaagagtgaagaagag660

cttgaggctattagcagggcactgtcattcttcagtttgtttgagcctaacattgaaaaa720

gtggaaggaacattccccaattcagaaatcgagatgctggaacagtttctttcaacacca780

gctgatgttgacttcatcactaagacactcaaagcaaaagaggtggaggcctatgctgat840

ctttgtgacagccactacctaaagcctgaaaaaaccattcaggagcggctagagatcaat900

agatgtgaggctattgataaaactcaggacctcctagctagcctgcatgcaaggagcaac960

aaacaaacatcactgaatcgagggacagtcaaactcccgccctggctaccaaagccatca1020

agtgagtcaatagacatcaagaccgactcaggctttggttccttaatggaccatggcgca1080

tatggtgagctgtgggcaaagtgccttctagatgtctcgctgggcaatgtggagggggta1140

atcagtgaccctgcaaaagaacttgacattgctatctctgatgatccagaaaaagatacc1200

cccaaagaagcaaagataacctataggcgattcaagcctgccttaagttcaagtgcccgt1260

caggaattttctctccaaggagtggagggaaagaagtggaagagaatggcagcaaaccag1320

aagaaagagaaggagtcccatgaggcattgagccccttcttggatgttgaggacattggg1380

gatttcctaacattcaacaatcttcttgcagattcaaggtatggagatgagtccgtccag1440

agagctgtgtcaatcctgttggagaaggcatctgccatgcaaaacacagagctaactcat1500

gccctcaatgactcattcaagaggaacctaagcagtaatgtggttcagtggtccctctgg1560

gtctcctgtttagcacaggagctagctagtgctctgaagcagcactgcagggctggtgag1620

ttcatcatcaagaagttgaagttctggcctatctatgtcatcatcaagccgaccaaatcg1680

tcttcccatatcttctacagcttagggatccgcaaggctgatgtgacaaggaggctcact1740

ggcagagtcttctctgacaccattgatgctggggaatgggagctaacagagttcaaaagc1800

ctgaagacatgcaagctcacgaaccttgtcaacttaccatgcaccatgctgaactcaata1860

gctttctggagagagaagctgggcgtggctccatggctggttcggaagccttgttcagag1920

ctcagagagcaggtgggcctgaccttcctgatcagtctggaggacaagtctaagactgag1980

gagatcatcaccttgacaaggtacacccagatggagggctttgtctctcctcccatgctg2040

cctaagccccaaaagatgctagggaaactggatggacctttgagaactaagttacaggta2100

tacctcctcaggaagcatctggattgcatggtgcgaattgcttctcagccattcagccta2160

atccctagagaggggagggtagaatggggaggaacattccatgccatctcaggccggtcc2220

acaaaccttgagaatatggtgaacagctggtacattgggtactacaagaacaaagaggag2280

tcaacagagctaaatgctctcggagaaatgtataagaagattgtagagatggaagaggac2340

aagcccagtagccctgagtttctagggtggggggacactgattcccctaagaagcatgaa2400

ttctcacggagcttcctcagagctgcttgctcatctctggagagagaaattgctcagcga2460

catggaagacaatggaagcagaaccttgaggagcgtgtcctgagagagattgggaccaag2520

aacatcctggaccttgcatccatgaaggctacaagcaacttttccaaagactgggagctc2580

tactcagaggtccagaccaaagagtaccataggtccaaactgctggagaagatggccaca2640

ttgatagagaagggggttatgtggtacattgatgctgtgggccaggcatggaaggcagtt2700

ctagatgacgggtgcatgcgaatctgtctcttcaaaaagaatcagcatggtggtctcaga2760

gagatctacgttatggatgcaaatgcccggctcgtgcagtttggggttgagaccatggct2820

aggtgtgtctgtgagttgagcccacatgagactgttgccaaccctagactcaagaattcc2880

atcatagagaaccatgggctgaagtcagcccgtagtcttggtcctggctctataaacata2940

aactcatccaatgacgccaagaagtggaatcaggggcactacacaacaaagctagctcta3000

gttctttgttggttcatgccagccaaattccacagattcatttgggccgccatttccatg3060

tttcggagaaaaaagatgatggtggacctaaggtttttagctcacctcagttctaaatct3120

gagtctaggtcatctgacccgtttagggaagcaatgacagacgctttccatggtaatagg3180

gaagtctcatggatggacaaagggcgaacttacataaagacagagacaggaatgatgcag3240

ggcatactgcactttacatccagcctccttcactcttgtgttcagagcttttacaagtct3300

tatttcgtctcgaagcttaaggagggctacatgggggaaagcatcaatggggtggtggat3360

gtcatagaaggctctgacgactctgcgatcatgatcagcatacgccctaagtcagacatg3420

gatgaagtccgatcaaggttctttgttgctaacttgctccactctgtcaagttcttgaac3480

cctttgtttggtatttactcatcagagaaatcaacagtgaacacagtgtattgtgttgag3540

tataactctgaattccacttccacaggcacttggtcagacccacactgagatggatagca3600

gcgtctcaccaaatctcagagactgaagcccttgcaagcaggcaagaggattactccaac3660

cttctaacccagtgcttggaaggaggggcctcattctctcttacctaccttatacagtgc3720

gctcagctcctgcaccactacatgctcctaggactatgcttacatcccttgtttggaact3780

ttcatggggatgctgatatcagacccagatccagccctagggttcttcctcatggacaac3840

cctgcattcgcagggggagcaggatttaggttcaatctgtggagggcctgcaagactaca3900

gaccttgggcggaagtatgcatattattttaatgagatacagggtaaaacaaagggagat3960

gaggattacagagctctggacgccacatcgggaggaactctcagccactctgtcatggtg4020

tactggggggacaggaagaagtatcaggccttattgaacaggatgggccttcctgaagac4080

tgggtggagcagatagatgagaatcctggagtcctttacaggagagctgccaacaaaaag4140

gaactactcttaaaactggcagagaaggttcattcaccaggtgtgactagcagcctgagt4200

aaagggcatgtagtgcctcgggtggtggcagcaggagtataccttctctcacgccactgc4260

tttcgctttagctcaagtatccatggaaggggctcagcacagaaggctagtcttataaaa4320

ctgctgatgatgtcttctatttctgccatgaagcacgggggctcactaaaccctaatcag4380

gagcgaatgctcttccctcaggcccaggagtatgacagagtatgcacattgcttgaggaa4440

gttgagcacctaacagggaaatttgttgttagggagagaaacattgtcaggagccgtata4500

gacttgttccaagagccagtggacttgcggtgcaaggcagaagatctggtgtcagaggtg4560

tggtttggcctgaaaaggactaagcttggaccccgtctcctcaaggaagagtgggacaaa4620

cttagggcctcatttgcatggctgagcacagacccatctgaaacattgagggatggccct4680

tttcttagccatgtgcagtttagaaacttcatagcccatgttgatgccaaatcaagatca4740

gtcaggctcctaggtgcccccgtaaagaagtcaggtggggtcaccactataagccaagta4800

gtcaggatgaacttcttccctggttttagcctagaagctgagaagagcttagacaatcag4860

gaaagacttgagagcatctccatcctcaagcatgtcttgttcatggtcttgaatggccca4920

tacactgaggagtacaagctggaaatgatcatagaggccttctctactcttgtgatacct4980

cagccatcagaggtcatcaggaaatcaaggaccatgactttatgcctcttatcgaattac5040

ttgtctagtaggggtgggtccattctagaccagattgagagggcacagtcaggcactcta5100

gggggcttcagcaagccccagaagacattcattaggccaggaggtggtattggctacaag5160

ggaaaaggtgtgtggactggagtgatggaggacacccatgttcaaatcttgatagatgga5220

gatgggactagtaactggcttgaggagatcaggctcagtagtgatgccaggctttatgat5280

gtcattgaatccatccgaaggttatgtgacgaccttgggatcaataacagggtggcatct5340

gcatatagaggtcattgcatggttaggctgagtgggttcaagatcaagccagcatcaagg5400

actgacggctgtccagtcaggattatggaaaggggcttcaggatcagggaacttcaaaac5460

ccagatgaggtcaagatgagagtgaggggtgacatcctcaacctttcagtcaccatacaa5520

gaaggaagggtcatgaacattctaagctacaggcctagagacactgatatatcagagtca5580

gccgcagcatacctctggagcaatcgagacctcttctcctttgggaagaaggagccatcc5640

tgcagctggatctgcttgaaaactcttgacaattgggcctggtcacatgcctcagttctc5700

ctggcaaatgataggaagacccaaggcattgataatagagccatggggaacattttcagg5760

gactgtctcgagggctctcttagaaagcaggggctgatgaggtcaaaactcacagagatg5820

gtggagaagaatgttgttcctttaacaactcaagagcttgtcgacatcttggaggaggac5880

atagacttttcagatgtcatagctgtggagctctcagagggatcacttgacattgaatcc5940

atctttgatggggcacctatcttgtggtctgctgaggtggaagagttcggggaaggagtg6000

gtggctgtgagctattccagtaagtactatcatctaaccctgatggatcaggctgccatc6060

acaatgtgtgcgatcatgggtaaggaaggctgtagagggctccttactgagaagagatgc6120

atggcagccatacgagagcaggtacggccattcctcatattcctgcaaattcctgaggac6180

agcatttcttgggtatctgatcagttctgtgactccaggggtcttgatgaagagagcacc6240

attatgtggggttga6255

<210>6

<211>3222

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2m基因

<400>6

atgatgaaagtcatctggttctcctctctgatctgcttagtcattcaatgcagtggggac60

acgggcccaatcatatgcgcagggcccatccactcaaacaagagtgccgacataccccac120

ctgcttggttactctgagaagatttgtcagatagatcggctgatacatgtttcgtcatgg180

ctgagaaaccattcacaatttcagggctacgtggggcagcgaggtggacgctctcaggtg240

agttacttcccagctgaaaactcttactcaaggtggagtgggcttctaagcccctgtgat300

gctgattggcttgggatgcttgtcgtgaagaaggccaaggggtctgatatgatagttcct360

gggccttcatacaagggaaaagtcttttttgaacggccaacttttgatggatatgttggc420

tggggctgtggcagtgggaagtcaaggactgagtcaggagagctctgcagctcagactca480

gggaccagttccggtcttctgccctcaaatagggttctctggataggtgatgttgcttgc540

cagcctatgacacccatccctgaagagacatttctggagctgaagagttttagccagagt600

gaatttccagatatatgcaaaattgatggcattgtgttcaaccagtgtgagagtgagagc660

ctacctcagccctttgatgttgcttggatggatgttggccactctcataaaatcatcatg720

agggagcacaagaccaaatgggtacaagagagctcatctaaggattttgtgtgctacaag780

gaagggactgggccgtgttctgaatcagaagaaaagacttgcaagaccagtggatcatgt840

aggggggacatgcagttttgcaaggtggcaggttgtgaacatggggaagagacatctgag900

gccaaatgcagatgctcactagtgcacaagcccggggaagttgttgtgtcatatggaggg960

atgcgtgtcagaccaaaatgctatggtttctccagaatgatggcaacactagaggtgaac1020

ccaccagagcaaaggattggccaatgtactggctgccatctagaatgcataaatgggggt1080

gtgaggataatcactctaactagtgagctcaagtcagctactgtctgtgcttcccacttt1140

tgtagttctgccacaagtggcaagaaaagcacggagattcaattccactcagggtcatta1200

gttgggaaaacagcaattcacgtcaaaggggcattggtggatggaactgaattcacattt1260

gaaggcagttgcatgttcccagatggttgtgacgcagtggactgcacattctgtcgtgag1320

tttctaaaaaatcctcagtgctaccctgcaaagaaatggttgttcatcattattgtcatc1380

ctccttggatatgcaggcctcatgctactcaccaatgtccttaaggcaatcggggtttgg1440

gggtcatgggtcatagctccagtgaagctaatgtttgccatcataaagaaactgatgaga1500

tctgtgagctgcttgatggggaaattgatggataggggaaggcaagtgatccatgaagaa1560

ataggggagaatagagagggcaaccaagatgatgttaggatcgagatggcaaggcctaga1620

agggtaaggcactggatgtactcacctgtcatcctgactattctagcaattgggcttgct1680

gaggggtgcgatgagatggtccatgcagattctaaacttgtttcgtgcaagcaagggagc1740

ggaaatatgaaggaatgtgtcacaactgggagggcgctccttcctgcggtgaacccaggg1800

caagaggcatgtctgcacttcacagcaccagggagtccggactcaaaatgtctcaaaatc1860

aaagttaagaggatcaacctgaaatgcaagaagtcatcatcatatttcgttcctgatgct1920

cggtccaggtgtacatctgtgaggagatgccgctgggcaggagactgtcagtctgggtgc1980

ccctctcatttcacgtccaactccttttctgacgattgggcaggtaaaatggacagggct2040

ggtctaggattcagtgggtgctctgatggatgtggaggagcagcctgcggctgctttaat2100

gcggccccttcatgcatcttctggaggaaatgggtagagaatccacatgggatcatctgg2160

aaagtatctccatgtgctgcatgggtcccatcagcagtcatagagcttacaatgccctcg2220

ggggaagtgaggacattccaccccatgagcggcatccctactcaagtcttcaagggtgtg2280

agtgtgacttatttgggctcagatatggaggtatctggcttgactgatctgtgtgaaata2340

gaagagctcaagtccaagaagctggcattagctccctgcaatcaggctggcatgggggtt2400

gtaggcaaggttggagagatacagtgcagtagtgaggaaagtgcccgtaccataaaaaaa2460

gatgggtgtatatggaatgctgacctcgtgggcatcgagctacgagtggatgacgctgtg2520

tgctactctaagatcactagtgtggaggcagttgcaaactactctgccatacccaccact2580

attgggggactgaggtttgagagaagccatgacagccagggcaaaatatctggtagcccc2640

ttggacatcacagccataagagggtctttttcagttaactatagaggccttcgactgagc2700

ctctcagaaattactgctacttgcacaggggaggtgacaaatgtgagtgggtgttactct2760

tgcatgacaggcgccaaagtctccatcaaactgcatagcagcaaaaatagcactgcacat2820

gtaaggtgcaaaggggatgagactgcgttcagtgtcctggagggagttcatagctatact2880

gtcagcctcagttttgaccatgcagtggtcgatgagcagtgccagctgaactgtgggggg2940

catgagagccaagtgactctaaaaggcaacctcatcttcctggatgtcccaaaattcgta3000

gatggcagctacatgcagacatatcatagctctgtacccacaggagcaaatatcccaagc3060

ccaacagactggctgaatgccctgtttggcaatgggctgagtaggtggattctgggggtg3120

ataggggttctactggggggattagctctctttttcctaatcatgtctttgttcaaactg3180

ggaacaaaacaggtatttcgatcaaggacgaagctggcttag3222

<210>7

<211>738

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2np基因

<400>7

atgtcagagtggtccaggattgcagtggagtttggtgagcagcagctcaatttgactgag60

cttgaagattttgcgagagagctggcctatgaaggccttgatcctgcattgatcatcaag120

aagctgaaggagacaggtggagatgattgggtgagggacacaaagttcatcattgtcttt180

gccctgactcgaggcaacaagatcgtcaaggcatcagggaaaatgtcaaactctgggtct240

aagaggttgatggcactccaagagaaatatgggctggttgagagggcagaaaccaggctc300

tcaatcactcctgtgagggttgcacagagccttcccacttggacatgtgctgcagcagca360

gccctaaaggagtatctcccagtggggccagctgtcatgaacctgaaggtcgaaaattat420

ccccctgagatgatgtgcatggcctttgggtccctgattccaactgcaggggtatctgaa480

gccacaacgaagaccctgatggaggcctactctctgtggcaagatgccttcaccaagact540

atcaatgtaaagatgcgcggagccagcaagacagaggtttacaactctttcagggatcct600

ctccatgctgctgtgaactctgtcttctttcccaatgatgtccgggtgaagtggctgaag660

gccaagggaatccttggcccagatggggtccccagcagagctgctgaggttgctgccgct720

gcttacagaaacctgtaa738

<210>8

<211>882

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2ns基因

<400>8

atgtcgctgagcaaatgctccaacgttgacctcaaatctgttgcaatgaatgccaacact60

gtcaggcttgagccatctctaggagagtaccccactcttaggagagacctcgttgaatgc120

tcttgtagtgtgttgactctatcaatggtcaagaggatgggcaagatgaccaacacagta180

tggctatttggcaacccaaagaatcctcttcaccagcttgagcctggactcgagcagctg240

ttagacatgtactacaaggacatgaggtgctactcccagagagagctgagtgctcttagg300

tggcctagtgggaagccatctgtatggttcctacaggcagctcacatgttcttctccatc360

aagaacagctgggcaatggaaaccggtagagagaactggcggggcctcttccacaggata420

acaaaaggccaaaagtatctttttgaaggggacatgatattggattctcttgaggccata480

gagaagcgaaggcttagactagggttacctgagatcctaataactgggctatccccaatt540

ctggatgtggccctcctccagatagagtcacttgcaaggctaagaggaatgagcttgaac600

caccacttattcacttcttcctcattgcgtaagcctctattagactgttgggacttcttt660

attcccatccgcaaaaagaagacagatggctcatacagtgtcctggatgaggatgatgag720

cctgggatccttcaaggttatccatatctgatggcacactatttgaataggtgcccattc780

cacaacctcatcaggtttgatgaagagctgagaactgcagccctaaacaccatctgggga840

agagattggccagccattggtgacctcccgaaggaggtctaa882

<210>9

<211>6255

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3l基因

<400>9

atgaacttggaagtgctttgtggtaggataaacgtggagaatgggctgtctcttggagaa60

ccaggcctgtacgaccaaatctacgacaggccagggctgccagacctagatgtgactgtc120

gatgccataggtgtgacagtggacataggggctgtgccagactcagcatcacaactgggt180

tcatcaatcaatgctgggttgatcacaatccagctctctgaagcatataagatcaatcat240

gatttcacgttttctggcctgtcaaagacaacagaccgacgcctctcagaggtattcccc300

attacacatgatggttctgatgggatgacccctgatgtgatccacaccagattggatgga360

accattgtggtggttgaattttcaactactaggagccataacattgggggtctggaggca420

gcatacaggacaaagatagaaaaatatagggaccctatctcaaggcgtgttgatatcatg480

gagaacccgagggtcttctttggcgtcattgtggtctcgtcagggggagttctgtccaac540

atgcccctgactcaggatgaggcagaggagctcatgtacaggttctgcatagccaatgag600

atctacactaaggctaggtctatggatgcagacattgagctgcagaagagcgaagaagag660

cttgaggctattagcagggcactatcattcttcagtttgtttgagccaaacattgaaaga720

gtggaaggaacattccctaattcggaaatcgagatgctggaacagtttctctcaactcca780

gctgatgttgacttcatcaccaagaccctcaaagcaaaagaggtggaggcctatgctgat840

ctttgtgacagccactatctaaagcctgaaaaaaccattcaggagcggctagagatcaat900

agatgtgaggctattgacaagactcaggacctcctagctagcctgcatgcaaggagcaac960

aagcaaacatcattgaatcgagggacagtcaagctcccgccctggctaccaaagccatca1020

agtgagtcaatagacatcaagactgactcaggctttggttccttaatggatcatggcaca1080

tatggagagctgtgggcaaagtgcctcctagatgtctcgctgggcaatgtggagggggta1140

gtcagtgaccctgcaaaagaacttgacattgctatctctgatgatccagaaaaagatacc1200

cccaaagaggcaaagataacctataggcggttcaagcctgccttgagctcaagtgcccgt1260

caagaattttctctccaaggagtggaggggaagaagtggaagagaatggcagcaaaccag1320

aagaaagagaaggagtcccatgagacattgagccctttcttggatgttgaagacattggg1380

gatttcctaacattcaacaatcttctcgcagattcgaggtatggagatgagtccgtccag1440

agagctgtgtcaatcttgttggaaaaggcatctgccatgcaagacacagagctcactcat1500

gccctcaacgactcattcaagaggaacctaagcagcaatgtggttcagtggtccctttgg1560

gtctcctgtctagcacaagagctagctagtgctctgaagcagcactgcagggctggtgag1620

ttcatcatcaagaagctgaagttctggcctatctatgtcattatcaagccgaccaaatcg1680

tcatcccatatcttctacagcttagggatccgcaaggctgatgtgacaaggaggctcact1740

ggcagagtcttctctgacaccattgatgctggggaatgggagctaacagagttcaagagc1800

ctgaagacatgcaagctcaccaaccttgtcaacttgccatgcaccatgctgaactcaata1860

gctttctggagagagaagctgggcgtggctccatggctggttcggaagccttgttcagag1920

ctcagagagcaggtgggcctgaccttcttgatcagtttggaggacaagtctaagactgag1980

gagatcatcaccttgacaaggtacactcaaatggagggcttcgtctctcctcctatgctg2040

cctaagccccaaaagatgctagggaaactggatgggcctttgagaactaagctacaggta2100

tacctcctcaggaagcatctggattgcatggtgcgaattgcttcccagccattcagcctg2160

atccctagagaggggagggtagaatggggagggacattccatgccatctcaggccggtcc2220

acaaaccttgagaatatggtgaacagctggtacattgggtactacaagaacaaagaggag2280

tcaacagagctaaatgctcttggagaaatgtataagaagattgtggagatggaagaggac2340

aagcccagcagccctaagtttctagggtggggggacactgattcccctaagaagcatgaa2400

ttctcacggagcttcctcagagctgcttgctcatctctggagagagaaattgctcagcga2460

catggaagacaatggaagcagaaccttgaggagcgtgtcctgagagagattggaaccaag2520

aacatcctagaccttgcatccatgaaggctacaagcaacttttccaaagactgggagctc2580

tactcagaagtccagaccaaagagtaccataggtccaaattgctggagaagatggccaca2640

ttgattgagaagggggttatgtggtacattgatgctgtgggccaggcttggaaggcagtt2700

ctagatgacgggtgcatgcgaatctgtctcttcaaaaagaatcagcatggtggcctcaga2760

gagatctacgttatggatgcaaatgcccggctcgtgcagtttggggttgagaccatggct2820

aggtgtgtctgtgagctgagcccacatgagactgttgccaatcctaggcttaagaattcc2880

atcatagagaaccatgggctgaagtcagcccgtagccttggccctggctctataaacata2940

aactcatccaatgatgccaagaagtggaatcaggggcactacacaacaaagctagctcta3000

gttctttgttggttcatgccagccaaattccacagattcatttgggctgccatttccatg3060

tttcggagaaaaaagatgatggtggacctaaggtttttagctcacctcagttctaaatct3120

gagtccaggtcatctgatccatttagggaagcaatgacagacgcattccatggaaatagg3180

gaagtctcatggatggacaaggggcgaacttacataaagacagagacagggatgatgcag3240

ggcatactgcactttacatccagcctcctccactcttgtgttcagagtttttacaagtct3300

tatttcgtctcgaagctcaaggagggctacatgggggaaagcatcagtggggtggtggat3360

gtcatagaaggttctgacgactcagcgatcatgatcagcatacgccctaagtcagatatg3420

gatgaagtccgatcaaggttttttgttgctaacctgctccactctgtcaaattcttgaac3480

cctttgtttgggatttactcatcagagaagtcaacagtgaacacagtgtattgtgtcgag3540

tataactctgaattccatttccacaggcacttggttagacccacactgagatggatagca3600

gcgtctcaccaaatctcagagactgaagcccttgcaagcaggcaagaggattactccaac3660

cttctaacccagtgcttggaaggaggggcctcattctctcttacctacctcatacagtgc3720

gctcagctcctacaccactacatgcttctaggactatgcttgcatcccttgtttggaacc3780

ttcatggggatgctgatatcagacccagatcctgccctaggattcttcctcatggacaac3840

cctgcattcgcaggaggagcaggatttagattcaatctgtggagagcctgcaagaccaca3900

gaccttgggcggaagtatgcatattacttcaatgagatacagggtaaaacaaagggagat3960

gaggactacagagctctggacgccacatcgggaggaactctcagccactctgttatggtg4020

tactggggggacaggaagaagtatcaggccttattgaacaggatgggccttcctgaagac4080

tgggtggagcagatagatgagaatcctggagtcctttacaggagagctgccaacaagaag4140

gaactactcttaaaactggcagagaaggttcattcacctggtgtgactagcagcctgagt4200

aaagggcatgtagtgcctcgggtggtggcagcaggagtataccttctctcacgccactgc4260

tttcgctttagctcaagcatccatggaaggggctcagcacagaaggctagtctcataaaa4320

ctgctgatgatgtcttctatttctgccatgaaacacgggggctcattaaaccccaatcag4380

gagcgaatgctcttccctcaggctcaagagtatgatagagtatgcacattgcttgaggaa4440

gttgaacacctaacagggaaatttgttgttagggagagaaacattgtcaggagccgcata4500

gacttgttccaagagccagttgacttgcggtgtaaggcagaagatctggtgtcagaggtg4560

tggtttggcctgaaaaggactaagcttggaccccgtctcctcaaggaagagtgggacaaa4620

cttagggcctcatttgcatggctgagcacagacccatctgaaacattgagggatggtcct4680

tttcttagccatgtgcagtttaggaacttcatagcccacgttgatgccaaatcaagatca4740

gtcaggctcctaggtgcccccgtgaagaagtcaggtggggtaaccaccataagccaagta4800

gtcagaatgaacttcttccctggttttagcctagaagctgagaagagcttagacaatcag4860

gagagacttgagagcatctccatcctcaagcatgtcttgttcatggtcttgaatggccca4920

tacactgaggagtacaagctggacatgatcatagaggccttctctactcttgtgatacct4980

cagccatcagaggtcatcaggaaatcaaggaccatgactttatgcctcttatcgaattac5040

ttgtctagtaggggtgggtccattctagaccagattgagagggcacagtcaggcactcta5100

gggggattcagcaagccccagaagacattcatcaggccaggaggtggtattggctacaag5160

ggaaaaggtgtgtggactggagtgatggaggacacccatgttcaaattctgatagatgga5220

gatgggactagcaactggcttgaggagatcaggctcagtagtgatgccaggctttatgat5280

gtcattgaatccatccggaggttatgtgatgaccttgggatcaacaacagggtggcatcg5340

gcatataggggtcattgcatggttagactgagtggattcaagatcaagccagcatcaagg5400

actgacggctgtccagttaggattatggaaaggggcttcaggatcagagagcttcaaaac5460

ccagatgaggtcaagatgagagtgaggggtgacattctcaacctctctgttaccatacaa5520

gaaggaagagtcatgaacattctgagctacaggccgagagacactgatatatcagagtca5580

gcagcagcatacctatggagcaatcgagacctcttctcctttgggaagaaggagccatcc5640

tgcagctggatctgcttgaaaactcttgacaattgggcctggtcacatgcctcagttctc5700

ctggcaaatgataggaagacccaaggcattgataatagagctatggggaacattttcagg5760

gactgtctcgagggttccctcagaaagcaagggctgatgaggtcaaagctcactgagatg5820

gtggagaagaatgtggttcctttaacaactcaagagcttgtcgatatcctggaggaggat5880

atagacttttcagatgtcatagctgtggagctctcagagggatcacttgacattgagtcc5940

atctttgatggagcacctatcttgtggtctgctgaggtggaagagtttggagaaggagtg6000

gtagctgtgagctattccagtaagtactatcatctaaccctgatggaccaagctgccatc6060

acaatgtgtgcgatcatgggtaaggagggctgtagagggctcctcactgagaagagatgc6120

atggcagccatacgagagcaggtaaggccattcctcatattcctgcaaatccctgaggac6180

agcatttcttgggtgtctgatcagttctgcgactccaggggtcttgatgaagagagcacc6240

attatgtggggttaa6255

<210>10

<211>3222

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3m基因

<400>10

atgatgaaagtcatctggttctcctctctgatctgcttagtcattcaatgcagtggggat60

acgggcccaatcatatgcgctgggcccatccactcaaacaagagcgccaacataccccac120

ctgcttggttactctgagaaaatttgtcagatagatcggcttatacatgtttcgtcatgg180

ctgagaaaccattcacaatttcagggctacgtggggcagcgaggtggacgctctcaggtg240

agttactacccagctgaaaactcttactcaaggtggagtggacttctaagcccctgtgat300

gctgattggcttgggatgcttgtcgtgaagaaggccaaggggtctgatatgatagttcct360

gggccttcatacaaagggaaagtcttctttgaacggccaacttttgatggatatgtgggc420

tggggctgtggtagtgggaagtcaaggacagagtcaggtgagctctgcagctcagactca480

gggaccagttctggtctactgccctcaaatagggttctctggataggtgatgttgcttgc540

cagcctatgacacccatccctgaggagacatttctggagctgaagagttttagccagagt600

gaattcccagacatatgcaaagttgatggcattgtgttcaaccagtgtgagagtgagagt660

ctacctcagccctttgatgttgcatggatggatgtaggccactctcataaaatcatcatg720

agggagcacaagaccaaatgggtacaagagagctcatctaaggattttgtgtgctacaag780

gaagggactgggccgtgttctgaatcagaagaaaagacttgcaagaccagtggatcatgc840

aggggggacatgcagttttgcaaggtagcaggttgtgaacatggggaagaggcatctgaa900

gccaaatgtagatgctcactagtgcacaagcccggggaagttgttgtgtcatatggaggg960

atgcgtgtcagaccaaagtgttatggtttctccagaatgatggcaacactagaggtgaac1020

ccaccagagcaaagaattggccaatgcactggctgccatctagaatgcataaatgggggt1080

gtgaggctaattactctaactagtgagctcaagtcagctactgtctgtgcttcccacttt1140

tgtagttctgctacaagtggcaagaaaagcacggagattcaattccactcagggtcatta1200

gttgggaaagcagcaattcacgtcaaagggactttggtggatggaactgaattcacattt1260

gagggcagttgcatgttcccagatggttgtgacgcagtggactgcacattctgtcgcgag1320

ttcctaaaaaatcctcagtgctaccctgcaaagaaatggttgttcataattattgccatc1380

ctccttggatatgcaggcctcatgctgctcaccaatgttcttaaggcaatcggggtttgg1440

ggatcatgggtcatagctccagtgaagctgatgtttgccatcataaagaaactgatgaga1500

tctgtgagctgcttgatggggaaattaatggataggggaaggcaagtgattcatgaagaa1560

ataggggagaatagagagggcaaccaagaagatgttagaattgagattgcaagacccaga1620

agggtgaggcattggatgtactcacctgtcatcctggctattctagcaattgggcttgct1680

gagggctgcgatgagatggtccatgcagattcaaaacttgtttcgtgcaggcaagggagc1740

ggaaatatgaaggaatgtgtcacaactgggagggcgcttcttcctgcggtgaacccagga1800

caagaggcatgtctgcacttcacggcacctggaagtccggactcaaaatgtctcaagatc1860

aaggttaagaggatcaacctaaaatgtaagaagtcatcatcatattttgttcctgatgct1920

cggtccagatgtacgtctgtgaggagatgtcgctgggcaggagactgtcagtctgggtgc1980

ccctctcatttcacgtccaactccttctctgatgattgggcaggtaaaatggacagggct2040

ggtctaggattcagtgggtgctctgatggatgtggaggagcagcctgcggctgctttaat2100

gcggccccttcatgcatcttttggaggaaatgggtagagaatccacatgggatcatctgg2160

aaagtatctccatgtgctgcatgggtcccttcaacagtcatagagctaacaatgccctcg2220

ggggaagtgaggacattccaccccatgagcggcatccccacacaagtcttcaagggtgtg2280

agtgtgacttacttaggctcagatatggaggtgtctggcctgactgacctgtgtgagata2340

gaagagctcaagtccaagaagctggcattagctccctgcaatcaggctggcatgggagtt2400

gtgggcaaggttggagagatacagtgcagtagcgaggaaagcgcccgttccataaagaaa2460

gatgggtgtatatggaatgctgaccttgtgggcatagagctacgagtggatgacgcagtg2520

tgctattctaagatcactagtgtggaggcagttgcaaactactctgccatacccaccact2580

attggggggttgaggtttgagagaagccatgacagccagggtaaaatatctggtagcccc2640

ctagacattacagccataagagggtctttttccgttaattatagaggccttcgactgagc2700

ctctcagaaatcactgctacttgcacaggagaggtgacgaatgtgagtgggtgttactct2760

tgcatgacaggcgccaaagtctccatcaaactgcatagcagcaaaaatagcactgcccat2820

gtaagatgcaaaggggatgagaccgcattcagtgtcctggagggagttcatagctatact2880

gtcagtcttagctttgaccatgcagtggtcgatgagcagtgccagctgaactgtgggggg2940

catgagagtcaagtgactctaaaaggcaacctcatcttcctggatgtcccaaaatttgta3000

gatggcagctatatgcagacatatcatagttctgtgcccacaggggctaatatcccaagc3060

cctacagactggctgaatgccttgtttggcaatgggctgagtaggtggattcttggggta3120

ataggggttctactggggggattggctctcttcttcttaattatgtccttgttcaaactg3180

ggaacaaaacagatattccgatcaaggacgaagctggcttag3222

<210>11

<211>738

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3np基因

<400>11

atgtcagagtggtccaggattgcagtggagtttggtgagcagcagctcaatttgactgag60

cttgaggatttcgcgagagagctagcctatgaaggccttgatcctgccttgataatcaag120

aagctgaaggagacaggtggagatgattgggtgagagatacaaagttcatcattgtcttt180

gccctgacccgaggcaacaagatcgtcaaggcatcagggaaaatgtcaaactcagggtct240

aagaggttgatggcacttcaagagaaatatggactggttgagagggcagaaaccaggctc300

tcaatcactcctgtgagggtagcacagagccttcccacttggacgtgcgctgcagcagca360

gccttaaaggagtatctcccagtgggcccagctgtcatgaacctgaaggtcgaaaattac420

cccccagagatgatgtgcatggcctttgggtctctgattccaactgcaggggtatcagaa480

gccacaacgaagaccctgatggaggcctactctctgtggcaagatgccttcaccaagact540

attaatgtgaagatgcgtggagccagcaagacagaggtttacaactccttcagagatccc600

ctccatgctgctgtgaactctgtcttctttcccaatgatgttcgggtgaagtggctgaag660

gccaagggaatcctaggcccagatggggtccccagcagagctgctgaggttgctgctgct720

gcttacagaaacctgtaa738

<210>12

<211>882

<212>dna

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3ns基因

<400>12

atgtcgctgagcaaatgctccaacgttgacctcaaatctgtagcaatgaatgccaacact60

gttaggcttgaaccatctctaggagagtaccccactcttaggaaagacctcgttgaatgc120

tcttgtagtgtgttgactctatcaatggtcaagaggatgggcaagatgaccaacacagta180

tggttatttggcaacccaaaaaatcctcttcaccagcttgagcctggacttgagcagctg240

ttggatatgtactacaaggacatgaggtgctactcccagagagagctgagtgccctcagg300

tggcctagtgggaagccttctgtatggttcctacaggcagctcacatgttcttttccatc360

aagaacagctgggcaatggaaaccggtagagagaactggcggggcctcttccacaggata420

acaaaaggcaaaaagtatctttttgaaggggacatgatattggattctcttgaagccata480

gagaagcgaaggctcagacttgggttacctgagatcctaataactggactatccccaatt540

ctggatgtggccctcctccagatagagtcacttgcaaggctaagaggcatgagcttgaac600

caccacttattcacttcttcctcactgcgtaagcctctgttagattgttgggacttcttt660

attcctatccgcaaaaagaagacagatggctcatacagtgttttggatgaggatgatgag720

cctggggtccttcaaggttacccatatctgatggcacactatctgaacaggtgcccattc780

cacaacctcatcagatttgatgaagaactgagaactgcagccctgaacaccatctgggga840

agagattggccggccattggtgacctcccgaaggaggtctaa882

<210>13

<211>2085

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1l

<400>13

metaspleugluvalleucysglyargileasnvalgluasnglyleu

151015

serleuglygluproglyleutyraspglniletyraspargprogly

202530

leuproaspleuaspvalthrvalaspalathrglyvalthrvalasp

354045

ileglyalavalproaspseralaserglnleuglyserserileasn

505560

alaglyleuilethrileglnleuserglualatyrlysileasnhis

65707580

aspphethrpheserglyleuserlysthrthraspargargleuser

859095

gluvalpheproilethrhisaspglyseraspglymetthrproasp

100105110

valilehisthrargleuaspglythrilevalvalvalglupheser

115120125

thrthrargserhisasnileglyglyleuglualaalatyrargthr

130135140

lysileglulystyrargaspproileserargargvalaspilemet

145150155160

gluasnproargvalphepheglyvalilevalvalserserglygly

165170175

valleuserasnmetproleuthrglnaspglualaglugluleumet

180185190

tyrargphecysilealaasngluiletyrthrlysalaargsermet

195200205

aspalaaspilegluleuglnlyssergluglugluleuglualaile

210215220

serargalaleuserphepheserleuphegluproasnilegluarg

225230235240

valgluglythrpheproasnsergluileglumetleugluglnphe

245250255

leuserthrproalaaspvalasppheilethrlysthrleulysala

260265270

lysgluvalglualatyralaaspleucysaspserhistyrleulys

275280285

proglulysthrileglngluargleugluileasnargcysgluala

290295300

ileasplysthrglnaspleuleualaglyleuhisalaargserasn

305310315320

lysglnthrserleuasnargglythrvallysleuproprotrpleu

325330335

prolysprosersergluserileaspilelysthraspserglyphe

340345350

glyserleumetasphisglyalatyrglygluleutrpalalyscys

355360365

leuleuaspvalserleuglyasnvalgluglyvalvalserasppro

370375380

alalysgluleuaspilealaileseraspaspproglulysaspthr

385390395400

prolysglualalysilethrtyrargargphelysproalaleuser

405410415

serseralaargglnglupheserleuglnglyvalgluglylyslys

420425430

trplysargmetalaalaasnglnlyslysglulysgluserhisglu

435440445

thrleuserpropheleuaspvalgluaspileglyasppheleuthr

450455460

pheasnasnleuleualaaspserargtyrglyaspgluserilegln

465470475480

argalavalserileleuleuglulysalaseralametglnaspthr

485490495

gluleuthrhisalaleuasnaspserphelysargasnleuserser

500505510

asnvalvalglntrpserleutrpvalsercysleualaglngluleu

515520525

alaseralaleulysglnhiscysargalaglyglupheileilelys

530535540

lysleulysphetrpproiletyrvalileilelysprothrlysser

545550555560

serserhisilephetyrserleuglyilearglysalaaspvalthr

565570575

argargleuthrglyargvalpheseraspthrileaspalaglyglu

580585590

trpgluleuthrgluphelysserleulysthrcyslysleuthrasn

595600605

leuvalasnleuprocysthrmetleuasnserilealaphetrparg

610615620

glulysleuglyvalalaprotrpleuvalarglysprocysserglu

625630635640

leuarggluglnvalglyleuthrpheleuileserleugluasplys

645650655

serlysthrglugluileilethrleuthrargtyrthrglnmetglu

660665670

glyphevalserproprometleuprolysproglnlysmetleugly

675680685

lysleuaspglyproleuargthrlysleuglnvaltyrleuleuarg

690695700

lyshisleuaspcysmetvalargilealaserglnpropheserleu

705710715720

ileproarggluglyargvalglutrpglyglythrphehisalaile

725730735

serglyargserthrasnleugluasnmetvalasnsertrptyrile

740745750

glytyrtyrlysasnlysglugluserthrgluleuasnalaleugly

755760765

glumettyrlyslysilevalglumetglugluasplysproserser

770775780

proglupheleuglytrpglyaspthraspserprolyslyshisglu

785790795800

pheserargserpheleuargalaalacysserserleugluargglu

805810815

ilealaglnarghisglyargglntrplysglnasnleuglugluarg

820825830

valleuarggluileglythrlysasnileleuaspleualasermet

835840845

lysalathrserasnpheserlysasptrpgluleutyrsergluval

850855860

glnthrlysglutyrhisargserlysleuleuglulysmetalathr

865870875880

leuileglulysglyvalmettrptyrileaspalavalglyglnala

885890895

trplysalavalleuaspaspglycysmetargilecysleuphelys

900905910

lysasnglnhisglyglyleuarggluiletyrvalmetaspalaasn

915920925

alaargleuvalglnpheglyvalgluthrmetalaargcysvalcys

930935940

gluleuserprohisgluthrvalalaasnproargleulysasnser

945950955960

ileilegluasnhisglyleulysseralaargserleuglyprogly

965970975

serileasnileasnserserasnaspalaarglystrpasnglngly

980985990

histyrthrthrlysleualaleuvalleucystrpphemetproala

99510001005

lysphehisargpheiletrpalaalailesermetpheargarglys

101010151020

lysmetmetvalaspleuargpheleualahisleuserserlysser

1025103010351040

gluserargserserasppropheargglualametthraspalaphe

104510501055

hisglyasnarggluvalsertrpmetasplysglyargthrtyrile

106010651070

lysthrgluthrglymetmetglnglyileleuhisphethrserser

107510801085

leuleuhissercysvalglnserphetyrlyssertyrphevalser

109010951100

lysleulysgluglytyrmetglygluserileasnglyvalvalasp

1105111011151120

valilegluglyseraspaspseralailemetileserileargpro

112511301135

lysseraspmetaspgluvalargserargphephevalalaasnleu

114011451150

leuhisservallyspheleuasnproleupheglyiletyrserser

115511601165

glulysserthrvalasnthrvaltyrcysvalglutyrasnserglu

117011751180

phehisphehisarghisleuvalargprothrleuargtrpileala

1185119011951200

alaserhisglnilesergluthrglualaleualaserargglnglu

120512101215

asptyrserasnleuleuthrglncysleugluglyglyalaserphe

122012251230

serleuthrtyrleuileglncysalaglnleuleuhishistyrmet

123512401245

leuleuglyleucysleuhisproleupheglythrphemetglymet

125012551260

leuileseraspproaspproalaleuglyphepheleumetaspasn

1265127012751280

proalaphealaglyglyalaglypheargpheasnleutrpargala

128512901295

cyslysthrthraspleuglyarglystyralatyrtyrpheasnglu

130013051310

ileglnglylysthrlysglyaspgluasptyrargalaleuaspala

131513201325

thrserglyglythrleuserhisservalmetvaltyrtrpglyasp

133013351340

arglyslystyrglnalaleuleuasnargmetglyleuprogluasp

1345135013551360

trpvalgluglnileaspgluasnproglyvalleutyrargargala

136513701375

alaasnlyslysgluleuleuleulysleualaglulysvalhisser

138013851390

proglyvalthrserserleuserlysglyhisvalvalproargval

139514001405

valalaalaglyvaltyrleuleuserarghiscyspheargpheser

141014151420

serserilehisglyargglyseralaglnlysalaserleuilelys

1425143014351440

leuleumetmetserservalseralametlyshisglyglyserleu

144514501455

asnproasnglngluargmetleupheproglnalaglnglutyrasp

146014651470

argvalcysthrleuleuglugluvalgluhisleuthrglylysphe

147514801485

valvalarggluargasnilevalargserargileaspleuphegln

149014951500

gluprovalaspleuargcyslysalagluaspleuvalsergluval

1505151015151520

trppheglyleulysargthrlysleuglyproargleuleulysglu

152515301535

glutrpasplysleuargalaserphealatrpleuserthrasppro

154015451550

sergluthrleuargaspglypropheleuserhisvalglnphearg

155515601565

asnpheilealahisvalaspalalysserargservalargleuleu

157015751580

glyalaprovallyslysserglyglyvalthrthrileserglnval

1585159015951600

valargmetasnphepheproglypheserleuglualaglulysser

160516101615

leuaspasnglngluargleugluserileserileleulyshisval

162016251630

leuphemetvalleuasnglyprotyrthrgluglutyrlysleuglu

163516401645

metileileglualapheserthrleuvalileproglnproserglu

165016551660

valilearglysserargthrmetthrleucysleuleuserasntyr

1665167016751680

leuserserargglyglyserileleuaspglnilegluargalagln

168516901695

serglythrleuglyglypheserlysproglnlysthrpheilearg

170017051710

proglyglyglyileglytyrlysglylysglyvaltrpthrglyval

171517201725

metgluaspthrhisvalglnileleuileaspglyaspglythrser

173017351740

asntrpleuglugluileargleuserseraspalaargleutyrasp

1745175017551760

valilegluserileargargleucysaspaspleuglyileasnasn

176517701775

argvalalaseralatyrargglyhiscysmetvalargleusergly

178017851790

phelysilelysproalaserargthraspglycysprovalargile

179518001805

metgluargglypheargilearggluleuglnasnproaspgluval

181018151820

lysmetargvalargglyaspileleuasnleuservalthrilegln

1825183018351840

gluglyargvalmetasnileleusertyrargproargaspthrasp

184518501855

ilesergluseralaalaalatyrleutrpserasnargaspleuphe

186018651870

serpheglylyslysgluprosercyssertrpilecysleulysthr

187518801885

leuaspasntrpalatrpserhisalaservalleuleualaasnasp

189018951900

arglysthrglnglyileaspasnlysalametglyasnilephearg

1905191019151920

aspcysleugluglyserleuarglysglnglyleumetargserlys

192519301935

leuthrglumetvalglulysasnvalvalproleuthrthrglnglu

194019451950

leuvalaspileleuglugluaspileasppheseraspvalileala

195519601965

valgluleusergluglyserleuaspilegluserilepheaspgly

197019751980

alaproileleutrpseralagluvalgluglupheglygluglyval

1985199019952000

valalavalsertyrserserlystyrtyrhisleuthrleumetasp

200520102015

glnalaalailethrmetcysalailemetglylysgluglycysarg

202020252030

glyleuleuthrglulysargcysmetalaalailearggluglnval

203520402045

argpropheleuilepheleuglnileprogluaspserilesertrp

205020552060

valseraspglnphecysaspserargglyleuaspglugluserthr

2065207020752080

ilemettrpgly***

2085

<210>14

<211>1074

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1m

<400>14

metmetlysvaliletrppheserserleuilecysleuvalilegln

151015

cysserglyaspthrglyproileilecysalaglyproilehisser

202530

asnlysseralaasnileprohisleuleuglytyrserglulysile

354045

cysglnileaspargleuilehisvalsersertrpleuargasnhis

505560

serglnpheglnglytyrvalglyglnargglyglyargserglnval

65707580

sertyrtyrproalagluasnsertyrserargtrpserglyleuleu

859095

serprocysaspalaasptrpleuglymetleuvalvallyslysala

100105110

lysglyseraspmetilevalproglyprosertyrlysglylysval

115120125

phephegluargprothrpheaspglytyrvalglytrpglycysgly

130135140

serglylysserargthrgluserglygluleucysserseraspser

145150155160

glythrserserglyleuleuproseraspargvalleutrpilegly

165170175

aspvalalacysglnprometthrproileproglugluthrpheleu

180185190

gluleulysserpheserglnserglupheproaspilecyslysile

195200205

aspglyilevalpheasnglncysglusergluserleuproglnpro

210215220

leuaspvalalatrpmetaspvalglyhisserhislysileilemet

225230235240

arggluhislysthrlystrpvalglngluserserserlysaspphe

245250255

valcystyrlysgluglythrglyprocysserglusergluglulys

260265270

thrcyslysthrserglysercysargglyaspmetglnphecyslys

275280285

valalaglycysgluhisglygluglualaseraspalalyscysarg

290295300

cysserleuvalhislysproglygluvalvalvalsertyrglygly

305310315320

metargvalargprolyscystyrglypheserargmetmetalathr

325330335

leugluvalasnproprogluglnargileglyglncysthrglycys

340345350

hisleuglucysileasnglyglyvalargleuilethrleuthrser

355360365

gluleulysseralathrvalcysalaserhisphecysserserala

370375380

thrserglylyslysserthrgluileglnphehisserglyserleu

385390395400

valglylysthrthrilehisvallysglyalaleuvalaspglythr

405410415

gluphethrphegluglysercysmetpheproaspglycysaspala

420425430

valaspcysthrphecysargglupheleulysasnproglncystyr

435440445

proalalyslystrpleupheileileilevalileleuleuglytyr

450455460

alaglyleumetleuleuthrasnvalleulysalaileglyvaltrp

465470475480

glysertrpvalilealaprovallysleumetphealaileilelys

485490495

lysleumetargservalsercysleumetglylysleumetasparg

500505510

glyargglnvalilehisglugluileglygluasnarggluglyasn

515520525

glnaspaspvalargileglumetalaargproargargvalarghis

530535540

trpmettyrserprovalileleuthrileleualaileglyleuala

545550555560

gluglycysaspglumetvalhisalaaspserlysleuvalsercys

565570575

lysglnglyserglyasnmetlysglucysvalthrthrglyargala

580585590

leuleuproalavalasnproglyglnglualacysleuhisphethr

595600605

alaproglyserproaspserlyscysleulysilelysvallysarg

610615620

ileasnleulyscyslyslyssersersertyrphevalproaspala

625630635640

argserargcysthrservalargargcysargtrpalaglyaspcys

645650655

glnserglycysproserhisphethrserasnserpheseraspasp

660665670

trpalaglylysmetaspargalaglyleuglypheserglycysser

675680685

aspglycysglyglyalaalacysglycyspheasnalaalaproser

690695700

cysilephetrparglystrpvalgluasnprohisglyileiletrp

705710715720

lysvalserprocysalaalatrpvalproseralavalilegluleu

725730735

thrmetproserglygluvalargthrphehisprometserglyile

740745750

prothrglnvalphelysglyvalservalthrtyrleuglyserasp

755760765

metgluvalserglyleuthraspleucysgluileglugluleulys

770775780

serlyslysleualaleualaprocysasnglnalaglymetglyval

785790795800

valglylysvalglygluileglncysserserglugluseralaarg

805810815

thrilelyslysaspglycysiletrpasnalaaspleuvalglyile

820825830

gluleuargvalaspaspalavalcystyrserlysilethrserval

835840845

glualavalalaasntyrseralaileprothrthrileglyglyleu

850855860

argphegluargserhisaspserglnglylysileserglyserpro

865870875880

leuaspilethralaileargglyserpheservalasntyrarggly

885890895

leuargleuserleusergluvalthralathrcysthrglygluval

900905910

thrasnvalserglycystyrsercysmetthrglyalalysvalser

915920925

ilelysleuhisserserlysasnserthralahisvalargcyslys

930935940

glyaspgluthralapheservalleuvalglyvalhissertyrthr

945950955960

valserleuserpheasphisalavalvalaspgluglncysglnleu

965970975

asncysglyglyhisgluserglnvalthrleulysglyasnleuile

980985990

pheleuaspvalprolysphevalaspglysertyrmetglnthrtyr

99510001005

hisserservalprothrglyalaasnileproserprothrasptrp

101010151020

leuasnalaleupheglyasnglyleuserargtrpileleuglyval

1025103010351040

ileglyvalleuleuglyglyleualaleuphepheleuilemetser

104510501055

leuphelysleuglythrlysglnvalpheargserargthrlysleu

106010651070

ala***

<210>15

<211>1074

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1np

<400>15

metmetlysvaliletrppheserserleuilecysleuvalilegln

151015

cysserglyaspthrglyproileilecysalaglyproilehisser

202530

asnlysseralaasnileprohisleuleuglytyrserglulysile

354045

cysglnileaspargleuilehisvalsersertrpleuargasnhis

505560

serglnpheglnglytyrvalglyglnargglyglyargserglnval

65707580

sertyrtyrproalagluasnsertyrserargtrpserglyleuleu

859095

serprocysaspalaasptrpleuglymetleuvalvallyslysala

100105110

lysglyseraspmetilevalproglyprosertyrlysglylysval

115120125

phephegluargprothrpheaspglytyrvalglytrpglycysgly

130135140

serglylysserargthrgluserglygluleucysserseraspser

145150155160

glythrserserglyleuleuproseraspargvalleutrpilegly

165170175

aspvalalacysglnprometthrproileproglugluthrpheleu

180185190

gluleulysserpheserglnserglupheproaspilecyslysile

195200205

aspglyilevalpheasnglncysglusergluserleuproglnpro

210215220

leuaspvalalatrpmetaspvalglyhisserhislysileilemet

225230235240

arggluhislysthrlystrpvalglngluserserserlysaspphe

245250255

valcystyrlysgluglythrglyprocysserglusergluglulys

260265270

thrcyslysthrserglysercysargglyaspmetglnphecyslys

275280285

valalaglycysgluhisglygluglualaseraspalalyscysarg

290295300

cysserleuvalhislysproglygluvalvalvalsertyrglygly

305310315320

metargvalargprolyscystyrglypheserargmetmetalathr

325330335

leugluvalasnproprogluglnargileglyglncysthrglycys

340345350

hisleuglucysileasnglyglyvalargleuilethrleuthrser

355360365

gluleulysseralathrvalcysalaserhisphecysserserala

370375380

thrserglylyslysserthrgluileglnphehisserglyserleu

385390395400

valglylysthrthrilehisvallysglyalaleuvalaspglythr

405410415

gluphethrphegluglysercysmetpheproaspglycysaspala

420425430

valaspcysthrphecysargglupheleulysasnproglncystyr

435440445

proalalyslystrpleupheileileilevalileleuleuglytyr

450455460

alaglyleumetleuleuthrasnvalleulysalaileglyvaltrp

465470475480

glysertrpvalilealaprovallysleumetphealaileilelys

485490495

lysleumetargservalsercysleumetglylysleumetasparg

500505510

glyargglnvalilehisglugluileglygluasnarggluglyasn

515520525

glnaspaspvalargileglumetalaargproargargvalarghis

530535540

trpmettyrserprovalileleuthrileleualaileglyleuala

545550555560

gluglycysaspglumetvalhisalaaspserlysleuvalsercys

565570575

lysglnglyserglyasnmetlysglucysvalthrthrglyargala

580585590

leuleuproalavalasnproglyglnglualacysleuhisphethr

595600605

alaproglyserproaspserlyscysleulysilelysvallysarg

610615620

ileasnleulyscyslyslyssersersertyrphevalproaspala

625630635640

argserargcysthrservalargargcysargtrpalaglyaspcys

645650655

glnserglycysproserhisphethrserasnserpheseraspasp

660665670

trpalaglylysmetaspargalaglyleuglypheserglycysser

675680685

aspglycysglyglyalaalacysglycyspheasnalaalaproser

690695700

cysilephetrparglystrpvalgluasnprohisglyileiletrp

705710715720

lysvalserprocysalaalatrpvalproseralavalilegluleu

725730735

thrmetproserglygluvalargthrphehisprometserglyile

740745750

prothrglnvalphelysglyvalservalthrtyrleuglyserasp

755760765

metgluvalserglyleuthraspleucysgluileglugluleulys

770775780

serlyslysleualaleualaprocysasnglnalaglymetglyval

785790795800

valglylysvalglygluileglncysserserglugluseralaarg

805810815

thrilelyslysaspglycysiletrpasnalaaspleuvalglyile

820825830

gluleuargvalaspaspalavalcystyrserlysilethrserval

835840845

glualavalalaasntyrseralaileprothrthrileglyglyleu

850855860

argphegluargserhisaspserglnglylysileserglyserpro

865870875880

leuaspilethralaileargglyserpheservalasntyrarggly

885890895

leuargleuserleusergluvalthralathrcysthrglygluval

900905910

thrasnvalserglycystyrsercysmetthrglyalalysvalser

915920925

ilelysleuhisserserlysasnserthralahisvalargcyslys

930935940

glyaspgluthralapheservalleuvalglyvalhissertyrthr

945950955960

valserleuserpheasphisalavalvalaspgluglncysglnleu

965970975

asncysglyglyhisgluserglnvalthrleulysglyasnleuile

980985990

pheleuaspvalprolysphevalaspglysertyrmetglnthrtyr

99510001005

hisserservalprothrglyalaasnileproserprothrasptrp

101010151020

leuasnalaleupheglyasnglyleuserargtrpileleuglyval

1025103010351040

ileglyvalleuleuglyglyleualaleuphepheleuilemetser

104510501055

leuphelysleuglythrlysglnvalpheargserargthrlysleu

106010651070

ala***

<210>16

<211>294

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-1ns

<400>16

metserleuserlyscysserasnvalaspleulysservalalamet

151015

asnalaasnthrvalargleugluproserleuglyglutyrprothr

202530

leuargargaspleuvalglucyssercysservalleuthrleuser

354045

metvallysargmetglylysmetthrasnthrvaltrpleuphegly

505560

asnprolysasnproleuhisglnleugluproglyleugluglnleu

65707580

leuaspmettyrtyrlysaspmetargcystyrserglnarggluleu

859095

seralaleuargtrpproserglylysproservaltrppheleugln

100105110

alaalahismetphepheserilelysasnsertrpalametgluthr

115120125

glyarggluasntrpargglyleuphehisargilethrlysglylys

130135140

lystyrleuphegluglyaspmetileleuaspserleuglualaile

145150155160

glulysargargleuargleuglyleuprogluileleuilethrgly

165170175

leuserproileleuaspvalalaleuleuglnilegluserleuala

180185190

argleuargglymetserleuasnhishisleuphethrserserser

195200205

leuarglysproleuleuaspcystrpaspphepheileproilearg

210215220

lyslysargthraspglysertyrserileleuaspgluaspaspglu

225230235240

leuglyvalleuglnglytyrprotyrleumetalahistyrleuasn

245250255

argcysprophehisasnleuileargpheaspglugluleuargthr

260265270

alaalaleuasnthriletrpglyargasptrpproalaileglyasp

275280285

leuprolysgluval***

290

<210>17

<211>2085

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2l

<400>17

metasnleugluvalleucysglyargileasnvalgluasnglyleu

151015

serleuglygluproglyleutyraspglniletyraspargprogly

202530

leuproaspleuaspvalthrvalaspalathrglyvalthrvalasp

354045

ileglyalavalproaspseralaserglnleuglyserserileasn

505560

alaglyleuilethrileglnleuserglualatyrlysileasnhis

65707580

aspphethrpheserglyleuserlysthrthraspargargleuser

859095

gluvalpheproilethrhisaspglyseraspglymetthrproasp

100105110

valilehisthrargleuaspglythrilevalvalvalglupheser

115120125

thrthrargserhisasnileglyglyleugluthralatyrargthr

130135140

lysileglulystyrargaspproileserargargvalaspilemet

145150155160

gluasnproargvalphepheglyvalilevalvalserserglygly

165170175

valleuserasnmetproleuthrglnaspglualaglugluleumet

180185190

tyrargphecysilealaasngluiletyrthrlysalaargsermet

195200205

aspalaaspilegluleuglnlyssergluglugluleuglualaile

210215220

serargalaleuserphepheserleuphegluproasnileglulys

225230235240

valgluglythrpheproasnsergluileglumetleugluglnphe

245250255

leuserthrproalaaspvalasppheilethrlysthrleulysala

260265270

lysgluvalglualatyralaaspleucysaspserhistyrleulys

275280285

proglulysthrileglngluargleugluileasnargcysgluala

290295300

ileasplysthrglnaspleuleualaserleuhisalaargserasn

305310315320

lysglnthrserleuasnargglythrvallysleuproprotrpleu

325330335

prolysprosersergluserileaspilelysthraspserglyphe

340345350

glyserleumetasphisglyalatyrglygluleutrpalalyscys

355360365

leuleuaspvalserleuglyasnvalgluglyvalileserasppro

370375380

alalysgluleuaspilealaileseraspaspproglulysaspthr

385390395400

prolysglualalysilethrtyrargargphelysproalaleuser

405410415

serseralaargglnglupheserleuglnglyvalgluglylyslys

420425430

trplysargmetalaalaasnglnlyslysglulysgluserhisglu

435440445

alaleuserpropheleuaspvalgluaspileglyasppheleuthr

450455460

pheasnasnleuleualaaspserargtyrglyaspgluservalgln

465470475480

argalavalserileleuleuglulysalaseralametglnasnthr

485490495

gluleuthrhisalaleuasnaspserphelysargasnleuserser

500505510

asnvalvalglntrpserleutrpvalsercysleualaglngluleu

515520525

alaseralaleulysglnhiscysargalaglyglupheileilelys

530535540

lysleulysphetrpproiletyrvalileilelysprothrlysser

545550555560

serserhisilephetyrserleuglyilearglysalaaspvalthr

565570575

argargleuthrglyargvalpheseraspthrileaspalaglyglu

580585590

trpgluleuthrgluphelysserleulysthrcyslysleuthrasn

595600605

leuvalasnleuprocysthrmetleuasnserilealaphetrparg

610615620

glulysleuglyvalalaprotrpleuvalarglysprocysserglu

625630635640

leuarggluglnvalglyleuthrpheleuileserleugluasplys

645650655

serlysthrglugluileilethrleuthrargtyrthrglnmetglu

660665670

glyphevalserproprometleuprolysproglnlysmetleugly

675680685

lysleuaspglyproleuargthrlysleuglnvaltyrleuleuarg

690695700

lyshisleuaspcysmetvalargilealaserglnpropheserleu

705710715720

ileproarggluglyargvalglutrpglyglythrphehisalaile

725730735

serglyargserthrasnleugluasnmetvalasnsertrptyrile

740745750

glytyrtyrlysasnlysglugluserthrgluleuasnalaleugly

755760765

glumettyrlyslysilevalglumetglugluasplysproserser

770775780

proglupheleuglytrpglyaspthraspserprolyslyshisglu

785790795800

pheserargserpheleuargalaalacysserserleugluargglu

805810815

ilealaglnarghisglyargglntrplysglnasnleuglugluarg

820825830

valleuarggluileglythrlysasnileleuaspleualasermet

835840845

lysalathrserasnpheserlysasptrpgluleutyrsergluval

850855860

glnthrlysglutyrhisargserlysleuleuglulysmetalathr

865870875880

leuileglulysglyvalmettrptyrileaspalavalglyglnala

885890895

trplysalavalleuaspaspglycysmetargilecysleuphelys

900905910

lysasnglnhisglyglyleuarggluiletyrvalmetaspalaasn

915920925

alaargleuvalglnpheglyvalgluthrmetalaargcysvalcys

930935940

gluleuserprohisgluthrvalalaasnproargleulysasnser

945950955960

ileilegluasnhisglyleulysseralaargserleuglyprogly

965970975

serileasnileasnserserasnaspalalyslystrpasnglngly

980985990

histyrthrthrlysleualaleuvalleucystrpphemetproala

99510001005

lysphehisargpheiletrpalaalailesermetpheargarglys

101010151020

lysmetmetvalaspleuargpheleualahisleuserserlysser

1025103010351040

gluserargserserasppropheargglualametthraspalaphe

104510501055

hisglyasnarggluvalsertrpmetasplysglyargthrtyrile

106010651070

lysthrgluthrglymetmetglnglyileleuhisphethrserser

107510801085

leuleuhissercysvalglnserphetyrlyssertyrphevalser

109010951100

lysleulysgluglytyrmetglygluserileasnglyvalvalasp

1105111011151120

valilegluglyseraspaspseralailemetileserileargpro

112511301135

lysseraspmetaspgluvalargserargphephevalalaasnleu

114011451150

leuhisservallyspheleuasnproleupheglyiletyrserser

115511601165

glulysserthrvalasnthrvaltyrcysvalglutyrasnserglu

117011751180

phehisphehisarghisleuvalargprothrleuargtrpileala

1185119011951200

alaserhisglnilesergluthrglualaleualaserargglnglu

120512101215

asptyrserasnleuleuthrglncysleugluglyglyalaserphe

122012251230

serleuthrtyrleuileglncysalaglnleuleuhishistyrmet

123512401245

leuleuglyleucysleuhisproleupheglythrphemetglymet

125012551260

leuileseraspproaspproalaleuglyphepheleumetaspasn

1265127012751280

proalaphealaglyglyalaglypheargpheasnleutrpargala

128512901295

cyslysthrthraspleuglyarglystyralatyrtyrpheasnglu

130013051310

ileglnglylysthrlysglyaspgluasptyrargalaleuaspala

131513201325

thrserglyglythrleuserhisservalmetvaltyrtrpglyasp

133013351340

arglyslystyrglnalaleuleuasnargmetglyleuprogluasp

1345135013551360

trpvalgluglnileaspgluasnproglyvalleutyrargargala

136513701375

alaasnlyslysgluleuleuleulysleualaglulysvalhisser

138013851390

proglyvalthrserserleuserlysglyhisvalvalproargval

139514001405

valalaalaglyvaltyrleuleuserarghiscyspheargpheser

141014151420

serserilehisglyargglyseralaglnlysalaserleuilelys

1425143014351440

leuleumetmetserserileseralametlyshisglyglyserleu

144514501455

asnproasnglngluargmetleupheproglnalaglnglutyrasp

146014651470

argvalcysthrleuleuglugluvalgluhisleuthrglylysphe

147514801485

valvalarggluargasnilevalargserargileaspleuphegln

149014951500

gluprovalaspleuargcyslysalagluaspleuvalsergluval

1505151015151520

trppheglyleulysargthrlysleuglyproargleuleulysglu

152515301535

glutrpasplysleuargalaserphealatrpleuserthrasppro

154015451550

sergluthrleuargaspglypropheleuserhisvalglnphearg

155515601565

asnpheilealahisvalaspalalysserargservalargleuleu

157015751580

glyalaprovallyslysserglyglyvalthrthrileserglnval

1585159015951600

valargmetasnphepheproglypheserleuglualaglulysser

160516101615

leuaspasnglngluargleugluserileserileleulyshisval

162016251630

leuphemetvalleuasnglyprotyrthrgluglutyrlysleuglu

163516401645

metileileglualapheserthrleuvalileproglnproserglu

165016551660

valilearglysserargthrmetthrleucysleuleuserasntyr

1665167016751680

leuserserargglyglyserileleuaspglnilegluargalagln

168516901695

serglythrleuglyglypheserlysproglnlysthrpheilearg

170017051710

proglyglyglyileglytyrlysglylysglyvaltrpthrglyval

171517201725

metgluaspthrhisvalglnileleuileaspglyaspglythrser

173017351740

asntrpleuglugluileargleuserseraspalaargleutyrasp

1745175017551760

valilegluserileargargleucysaspaspleuglyileasnasn

176517701775

argvalalaseralatyrargglyhiscysmetvalargleusergly

178017851790

phelysilelysproalaserargthraspglycysprovalargile

179518001805

metgluargglypheargilearggluleuglnasnproaspgluval

181018151820

lysmetargvalargglyaspileleuasnleuservalthrilegln

1825183018351840

gluglyargvalmetasnileleusertyrargproargaspthrasp

184518501855

ilesergluseralaalaalatyrleutrpserasnargaspleuphe

186018651870

serpheglylyslysgluprosercyssertrpilecysleulysthr

187518801885

leuaspasntrpalatrpserhisalaservalleuleualaasnasp

189018951900

arglysthrglnglyileaspasnargalametglyasnilephearg

1905191019151920

aspcysleugluglyserleuarglysglnglyleumetargserlys

192519301935

leuthrglumetvalglulysasnvalvalproleuthrthrglnglu

194019451950

leuvalaspileleuglugluaspileasppheseraspvalileala

195519601965

valgluleusergluglyserleuaspilegluserilepheaspgly

197019751980

alaproileleutrpseralagluvalgluglupheglygluglyval

1985199019952000

valalavalsertyrserserlystyrtyrhisleuthrleumetasp

200520102015

glnalaalailethrmetcysalailemetglylysgluglycysarg

202020252030

glyleuleuthrglulysargcysmetalaalailearggluglnval

203520402045

argpropheleuilepheleuglnileprogluaspserilesertrp

205020552060

valseraspglnphecysaspserargglyleuaspglugluserthr

2065207020752080

ilemettrpgly***

2085

<210>18

<211>1074

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2m

<400>18

metmetlysvaliletrppheserserleuilecysleuvalilegln

151015

cysserglyaspthrglyproileilecysalaglyproilehisser

202530

asnlysseralaaspileprohisleuleuglytyrserglulysile

354045

cysglnileaspargleuilehisvalsersertrpleuargasnhis

505560

serglnpheglnglytyrvalglyglnargglyglyargserglnval

65707580

sertyrpheproalagluasnsertyrserargtrpserglyleuleu

859095

serprocysaspalaasptrpleuglymetleuvalvallyslysala

100105110

lysglyseraspmetilevalproglyprosertyrlysglylysval

115120125

phephegluargprothrpheaspglytyrvalglytrpglycysgly

130135140

serglylysserargthrgluserglygluleucysserseraspser

145150155160

glythrserserglyleuleuproserasnargvalleutrpilegly

165170175

aspvalalacysglnprometthrproileproglugluthrpheleu

180185190

gluleulysserpheserglnserglupheproaspilecyslysile

195200205

aspglyilevalpheasnglncysglusergluserleuproglnpro

210215220

pheaspvalalatrpmetaspvalglyhisserhislysileilemet

225230235240

arggluhislysthrlystrpvalglngluserserserlysaspphe

245250255

valcystyrlysgluglythrglyprocysserglusergluglulys

260265270

thrcyslysthrserglysercysargglyaspmetglnphecyslys

275280285

valalaglycysgluhisglyglugluthrserglualalyscysarg

290295300

cysserleuvalhislysproglygluvalvalvalsertyrglygly

305310315320

metargvalargprolyscystyrglypheserargmetmetalathr

325330335

leugluvalasnproprogluglnargileglyglncysthrglycys

340345350

hisleuglucysileasnglyglyvalargileilethrleuthrser

355360365

gluleulysseralathrvalcysalaserhisphecysserserala

370375380

thrserglylyslysserthrgluileglnphehisserglyserleu

385390395400

valglylysthralailehisvallysglyalaleuvalaspglythr

405410415

gluphethrphegluglysercysmetpheproaspglycysaspala

420425430

valaspcysthrphecysargglupheleulysasnproglncystyr

435440445

proalalyslystrpleupheileileilevalileleuleuglytyr

450455460

alaglyleumetleuleuthrasnvalleulysalaileglyvaltrp

465470475480

glysertrpvalilealaprovallysleumetphealaileilelys

485490495

lysleumetargservalsercysleumetglylysleumetasparg

500505510

glyargglnvalilehisglugluileglygluasnarggluglyasn

515520525

glnaspaspvalargileglumetalaargproargargvalarghis

530535540

trpmettyrserprovalileleuthrileleualaileglyleuala

545550555560

gluglycysaspglumetvalhisalaaspserlysleuvalsercys

565570575

lysglnglyserglyasnmetlysglucysvalthrthrglyargala

580585590

leuleuproalavalasnproglyglnglualacysleuhisphethr

595600605

alaproglyserproaspserlyscysleulysilelysvallysarg

610615620

ileasnleulyscyslyslyssersersertyrphevalproaspala

625630635640

argserargcysthrservalargargcysargtrpalaglyaspcys

645650655

glnserglycysproserhisphethrserasnserpheseraspasp

660665670

trpalaglylysmetaspargalaglyleuglypheserglycysser

675680685

aspglycysglyglyalaalacysglycyspheasnalaalaproser

690695700

cysilephetrparglystrpvalgluasnprohisglyileiletrp

705710715720

lysvalserprocysalaalatrpvalproseralavalilegluleu

725730735

thrmetproserglygluvalargthrphehisprometserglyile

740745750

prothrglnvalphelysglyvalservalthrtyrleuglyserasp

755760765

metgluvalserglyleuthraspleucysgluileglugluleulys

770775780

serlyslysleualaleualaprocysasnglnalaglymetglyval

785790795800

valglylysvalglygluileglncysserserglugluseralaarg

805810815

thrilelyslysaspglycysiletrpasnalaaspleuvalglyile

820825830

gluleuargvalaspaspalavalcystyrserlysilethrserval

835840845

glualavalalaasntyrseralaileprothrthrileglyglyleu

850855860

argphegluargserhisaspserglnglylysileserglyserpro

865870875880

leuaspilethralaileargglyserpheservalasntyrarggly

885890895

leuargleuserleusergluilethralathrcysthrglygluval

900905910

thrasnvalserglycystyrsercysmetthrglyalalysvalser

915920925

ilelysleuhisserserlysasnserthralahisvalargcyslys

930935940

glyaspgluthralapheservalleugluglyvalhissertyrthr

945950955960

valserleuserpheasphisalavalvalaspgluglncysglnleu

965970975

asncysglyglyhisgluserglnvalthrleulysglyasnleuile

980985990

pheleuaspvalprolysphevalaspglysertyrmetglnthrtyr

99510001005

hisserservalprothrglyalaasnileproserprothrasptrp

101010151020

leuasnalaleupheglyasnglyleuserargtrpileleuglyval

1025103010351040

ileglyvalleuleuglyglyleualaleuphepheleuilemetser

104510501055

leuphelysleuglythrlysglnvalpheargserargthrlysleu

106010651070

ala***

<210>19

<211>246

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2np

<400>19

metserglutrpserargilealavalglupheglygluglnglnleu

151015

asnleuthrgluleugluaspphealaarggluleualatyrglugly

202530

leuaspproalaleuileilelyslysleulysgluthrglyglyasp

354045

asptrpvalargaspthrlyspheileilevalphealaleuthrarg

505560

glyasnlysilevallysalaserglylysmetserasnserglyser

65707580

lysargleumetalaleuglnglulystyrglyleuvalgluargala

859095

gluthrargleuserilethrprovalargvalalaglnserleupro

100105110

thrtrpthrcysalaalaalaalaalaleulysglutyrleuproval

115120125

glyproalavalmetasnleulysvalgluasntyrproproglumet

130135140

metcysmetalapheglyserleuileprothralaglyvalserglu

145150155160

alathrthrlysthrleumetglualatyrserleutrpglnaspala

165170175

phethrlysthrileasnvallysmetargglyalaserlysthrglu

180185190

valtyrasnserpheargaspproleuhisalaalavalasnserval

195200205

phepheproasnaspvalargvallystrpleulysalalysglyile

210215220

leuglyproaspglyvalproserargalaalagluvalalaalaala

225230235240

alatyrargasnleu***

245

<210>20

<211>294

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-2ns

<400>20

metserleuserlyscysserasnvalaspleulysservalalamet

151015

asnalaasnthrvalargleugluproserleuglyglutyrprothr

202530

leuargargaspleuvalglucyssercysservalleuthrleuser

354045

metvallysargmetglylysmetthrasnthrvaltrpleuphegly

505560

asnprolysasnproleuhisglnleugluproglyleugluglnleu

65707580

leuaspmettyrtyrlysaspmetargcystyrserglnarggluleu

859095

seralaleuargtrpproserglylysproservaltrppheleugln

100105110

alaalahismetphepheserilelysasnsertrpalametgluthr

115120125

glyarggluasntrpargglyleuphehisargilethrlysglygln

130135140

lystyrleuphegluglyaspmetileleuaspserleuglualaile

145150155160

glulysargargleuargleuglyleuprogluileleuilethrgly

165170175

leuserproileleuaspvalalaleuleuglnilegluserleuala

180185190

argleuargglymetserleuasnhishisleuphethrserserser

195200205

leuarglysproleuleuaspcystrpaspphepheileproilearg

210215220

lyslyslysthraspglysertyrservalleuaspgluaspaspglu

225230235240

proglyileleuglnglytyrprotyrleumetalahistyrleuasn

245250255

argcysprophehisasnleuileargpheaspglugluleuargthr

260265270

alaalaleuasnthriletrpglyargasptrpproalaileglyasp

275280285

leuprolysgluval***

290

<210>21

<211>2085

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3l

<400>21

metasnleugluvalleucysglyargileasnvalgluasnglyleu

151015

serleuglygluproglyleutyraspglniletyraspargprogly

202530

leuproaspleuaspvalthrvalaspalaileglyvalthrvalasp

354045

ileglyalavalproaspseralaserglnleuglyserserileasn

505560

alaglyleuilethrileglnleuserglualatyrlysileasnhis

65707580

aspphethrpheserglyleuserlysthrthraspargargleuser

859095

gluvalpheproilethrhisaspglyseraspglymetthrproasp

100105110

valilehisthrargleuaspglythrilevalvalvalglupheser

115120125

thrthrargserhisasnileglyglyleuglualaalatyrargthr

130135140

lysileglulystyrargaspproileserargargvalaspilemet

145150155160

gluasnproargvalphepheglyvalilevalvalserserglygly

165170175

valleuserasnmetproleuthrglnaspglualaglugluleumet

180185190

tyrargphecysilealaasngluiletyrthrlysalaargsermet

195200205

aspalaaspilegluleuglnlyssergluglugluleuglualaile

210215220

serargalaleuserphepheserleuphegluproasnilegluarg

225230235240

valgluglythrpheproasnsergluileglumetleugluglnphe

245250255

leuserthrproalaaspvalasppheilethrlysthrleulysala

260265270

lysgluvalglualatyralaaspleucysaspserhistyrleulys

275280285

proglulysthrileglngluargleugluileasnargcysgluala

290295300

ileasplysthrglnaspleuleualaserleuhisalaargserasn

305310315320

lysglnthrserleuasnargglythrvallysleuproprotrpleu

325330335

prolysprosersergluserileaspilelysthraspserglyphe

340345350

glyserleumetasphisglythrtyrglygluleutrpalalyscys

355360365

leuleuaspvalserleuglyasnvalgluglyvalvalserasppro

370375380

alalysgluleuaspilealaileseraspaspproglulysaspthr

385390395400

prolysglualalysilethrtyrargargphelysproalaleuser

405410415

serseralaargglnglupheserleuglnglyvalgluglylyslys

420425430

trplysargmetalaalaasnglnlyslysglulysgluserhisglu

435440445

thrleuserpropheleuaspvalgluaspileglyasppheleuthr

450455460

pheasnasnleuleualaaspserargtyrglyaspgluservalgln

465470475480

argalavalserileleuleuglulysalaseralametglnaspthr

485490495

gluleuthrhisalaleuasnaspserphelysargasnleuserser

500505510

asnvalvalglntrpserleutrpvalsercysleualaglngluleu

515520525

alaseralaleulysglnhiscysargalaglyglupheileilelys

530535540

lysleulysphetrpproiletyrvalileilelysprothrlysser

545550555560

serserhisilephetyrserleuglyilearglysalaaspvalthr

565570575

argargleuthrglyargvalpheseraspthrileaspalaglyglu

580585590

trpgluleuthrgluphelysserleulysthrcyslysleuthrasn

595600605

leuvalasnleuprocysthrmetleuasnserilealaphetrparg

610615620

glulysleuglyvalalaprotrpleuvalarglysprocysserglu

625630635640

leuarggluglnvalglyleuthrpheleuileserleugluasplys

645650655

serlysthrglugluileilethrleuthrargtyrthrglnmetglu

660665670

glyphevalserproprometleuprolysproglnlysmetleugly

675680685

lysleuaspglyproleuargthrlysleuglnvaltyrleuleuarg

690695700

lyshisleuaspcysmetvalargilealaserglnpropheserleu

705710715720

ileproarggluglyargvalglutrpglyglythrphehisalaile

725730735

serglyargserthrasnleugluasnmetvalasnsertrptyrile

740745750

glytyrtyrlysasnlysglugluserthrgluleuasnalaleugly

755760765

glumettyrlyslysilevalglumetglugluasplysproserser

770775780

prolyspheleuglytrpglyaspthraspserprolyslyshisglu

785790795800

pheserargserpheleuargalaalacysserserleugluargglu

805810815

ilealaglnarghisglyargglntrplysglnasnleuglugluarg

820825830

valleuarggluileglythrlysasnileleuaspleualasermet

835840845

lysalathrserasnpheserlysasptrpgluleutyrsergluval

850855860

glnthrlysglutyrhisargserlysleuleuglulysmetalathr

865870875880

leuileglulysglyvalmettrptyrileaspalavalglyglnala

885890895

trplysalavalleuaspaspglycysmetargilecysleuphelys

900905910

lysasnglnhisglyglyleuarggluiletyrvalmetaspalaasn

915920925

alaargleuvalglnpheglyvalgluthrmetalaargcysvalcys

930935940

gluleuserprohisgluthrvalalaasnproargleulysasnser

945950955960

ileilegluasnhisglyleulysseralaargserleuglyprogly

965970975

serileasnileasnserserasnaspalalyslystrpasnglngly

980985990

histyrthrthrlysleualaleuvalleucystrpphemetproala

99510001005

lysphehisargpheiletrpalaalailesermetpheargarglys

101010151020

lysmetmetvalaspleuargpheleualahisleuserserlysser

1025103010351040

gluserargserserasppropheargglualametthraspalaphe

104510501055

hisglyasnarggluvalsertrpmetasplysglyargthrtyrile

106010651070

lysthrgluthrglymetmetglnglyileleuhisphethrserser

107510801085

leuleuhissercysvalglnserphetyrlyssertyrphevalser

109010951100

lysleulysgluglytyrmetglygluserileserglyvalvalasp

1105111011151120

valilegluglyseraspaspseralailemetileserileargpro

112511301135

lysseraspmetaspgluvalargserargphephevalalaasnleu

114011451150

leuhisservallyspheleuasnproleupheglyiletyrserser

115511601165

glulysserthrvalasnthrvaltyrcysvalglutyrasnserglu

117011751180

phehisphehisarghisleuvalargprothrleuargtrpileala

1185119011951200

alaserhisglnilesergluthrglualaleualaserargglnglu

120512101215

asptyrserasnleuleuthrglncysleugluglyglyalaserphe

122012251230

serleuthrtyrleuileglncysalaglnleuleuhishistyrmet

123512401245

leuleuglyleucysleuhisproleupheglythrphemetglymet

125012551260

leuileseraspproaspproalaleuglyphepheleumetaspasn

1265127012751280

proalaphealaglyglyalaglypheargpheasnleutrpargala

128512901295

cyslysthrthraspleuglyarglystyralatyrtyrpheasnglu

130013051310

ileglnglylysthrlysglyaspgluasptyrargalaleuaspala

131513201325

thrserglyglythrleuserhisservalmetvaltyrtrpglyasp

133013351340

arglyslystyrglnalaleuleuasnargmetglyleuprogluasp

1345135013551360

trpvalgluglnileaspgluasnproglyvalleutyrargargala

136513701375

alaasnlyslysgluleuleuleulysleualaglulysvalhisser

138013851390

proglyvalthrserserleuserlysglyhisvalvalproargval

139514001405

valalaalaglyvaltyrleuleuserarghiscyspheargpheser

141014151420

serserilehisglyargglyseralaglnlysalaserleuilelys

1425143014351440

leuleumetmetserserileseralametlyshisglyglyserleu

144514501455

asnproasnglngluargmetleupheproglnalaglnglutyrasp

146014651470

argvalcysthrleuleuglugluvalgluhisleuthrglylysphe

147514801485

valvalarggluargasnilevalargserargileaspleuphegln

149014951500

gluprovalaspleuargcyslysalagluaspleuvalsergluval

1505151015151520

trppheglyleulysargthrlysleuglyproargleuleulysglu

152515301535

glutrpasplysleuargalaserphealatrpleuserthrasppro

154015451550

sergluthrleuargaspglypropheleuserhisvalglnphearg

155515601565

asnpheilealahisvalaspalalysserargservalargleuleu

157015751580

glyalaprovallyslysserglyglyvalthrthrileserglnval

1585159015951600

valargmetasnphepheproglypheserleuglualaglulysser

160516101615

leuaspasnglngluargleugluserileserileleulyshisval

162016251630

leuphemetvalleuasnglyprotyrthrgluglutyrlysleuasp

163516401645

metileileglualapheserthrleuvalileproglnproserglu

165016551660

valilearglysserargthrmetthrleucysleuleuserasntyr

1665167016751680

leuserserargglyglyserileleuaspglnilegluargalagln

168516901695

serglythrleuglyglypheserlysproglnlysthrpheilearg

170017051710

proglyglyglyileglytyrlysglylysglyvaltrpthrglyval

171517201725

metgluaspthrhisvalglnileleuileaspglyaspglythrser

173017351740

asntrpleuglugluileargleuserseraspalaargleutyrasp

1745175017551760

valilegluserileargargleucysaspaspleuglyileasnasn

176517701775

argvalalaseralatyrargglyhiscysmetvalargleusergly

178017851790

phelysilelysproalaserargthraspglycysprovalargile

179518001805

metgluargglypheargilearggluleuglnasnproaspgluval

181018151820

lysmetargvalargglyaspileleuasnleuservalthrilegln

1825183018351840

gluglyargvalmetasnileleusertyrargproargaspthrasp

184518501855

ilesergluseralaalaalatyrleutrpserasnargaspleuphe

186018651870

serpheglylyslysgluprosercyssertrpilecysleulysthr

187518801885

leuaspasntrpalatrpserhisalaservalleuleualaasnasp

189018951900

arglysthrglnglyileaspasnargalametglyasnilephearg

1905191019151920

aspcysleugluglyserleuarglysglnglyleumetargserlys

192519301935

leuthrglumetvalglulysasnvalvalproleuthrthrglnglu

194019451950

leuvalaspileleuglugluaspileasppheseraspvalileala

195519601965

valgluleusergluglyserleuaspilegluserilepheaspgly

197019751980

alaproileleutrpseralagluvalgluglupheglygluglyval

1985199019952000

valalavalsertyrserserlystyrtyrhisleuthrleumetasp

200520102015

glnalaalailethrmetcysalailemetglylysgluglycysarg

202020252030

glyleuleuthrglulysargcysmetalaalailearggluglnval

203520402045

argpropheleuilepheleuglnileprogluaspserilesertrp

205020552060

valseraspglnphecysaspserargglyleuaspglugluserthr

2065207020752080

ilemettrpgly***

2085

<210>22

<211>1074

<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3m

<400>22

metmetlysvaliletrppheserserleuilecysleuvalilegln

151015

cysserglyaspthrglyproileilecysalaglyproilehisser

202530

asnlysseralaasnileprohisleuleuglytyrserglulysile

354045

cysglnileaspargleuilehisvalsersertrpleuargasnhis

505560

serglnpheglnglytyrvalglyglnargglyglyargserglnval

65707580

sertyrtyrproalagluasnsertyrserargtrpserglyleuleu

859095

serprocysaspalaasptrpleuglymetleuvalvallyslysala

100105110

lysglyseraspmetilevalproglyprosertyrlysglylysval

115120125

phephegluargprothrpheaspglytyrvalglytrpglycysgly

130135140

serglylysserargthrgluserglygluleucysserseraspser

145150155160

glythrserserglyleuleuproserasnargvalleutrpilegly

165170175

aspvalalacysglnprometthrproileproglugluthrpheleu

180185190

gluleulysserpheserglnserglupheproaspilecyslysval

195200205

aspglyilevalpheasnglncysglusergluserleuproglnpro

210215220

pheaspvalalatrpmetaspvalglyhisserhislysileilemet

225230235240

arggluhislysthrlystrpvalglngluserserserlysaspphe

245250255

valcystyrlysgluglythrglyprocysserglusergluglulys

260265270

thrcyslysthrserglysercysargglyaspmetglnphecyslys

275280285

valalaglycysgluhisglygluglualaserglualalyscysarg

290295300

cysserleuvalhislysproglygluvalvalvalsertyrglygly

305310315320

metargvalargprolyscystyrglypheserargmetmetalathr

325330335

leugluvalasnproprogluglnargileglyglncysthrglycys

340345350

hisleuglucysileasnglyglyvalargleuilethrleuthrser

355360365

gluleulysseralathrvalcysalaserhisphecysserserala

370375380

thrserglylyslysserthrgluileglnphehisserglyserleu

385390395400

valglylysalaalailehisvallysglythrleuvalaspglythr

405410415

gluphethrphegluglysercysmetpheproaspglycysaspala

420425430

valaspcysthrphecysargglupheleulysasnproglncystyr

435440445

proalalyslystrpleupheileileilealaileleuleuglytyr

450455460

alaglyleumetleuleuthrasnvalleulysalaileglyvaltrp

465470475480

glysertrpvalilealaprovallysleumetphealaileilelys

485490495

lysleumetargservalsercysleumetglylysleumetasparg

500505510

glyargglnvalilehisglugluileglygluasnarggluglyasn

515520525

glngluaspvalargilegluilealaargproargargvalarghis

530535540

trpmettyrserprovalileleualaileleualaileglyleuala

545550555560

gluglycysaspglumetvalhisalaaspserlysleuvalsercys

565570575

argglnglyserglyasnmetlysglucysvalthrthrglyargala

580585590

leuleuproalavalasnproglyglnglualacysleuhisphethr

595600605

alaproglyserproaspserlyscysleulysilelysvallysarg

610615620

ileasnleulyscyslyslyssersersertyrphevalproaspala

625630635640

argserargcysthrservalargargcysargtrpalaglyaspcys

645650655

glnserglycysproserhisphethrserasnserpheseraspasp

660665670

trpalaglylysmetaspargalaglyleuglypheserglycysser

675680685

aspglycysglyglyalaalacysglycyspheasnalaalaproser

690695700

cysilephetrparglystrpvalgluasnprohisglyileiletrp

705710715720

lysvalserprocysalaalatrpvalproserthrvalilegluleu

725730735

thrmetproserglygluvalargthrphehisprometserglyile

740745750

prothrglnvalphelysglyvalservalthrtyrleuglyserasp

755760765

metgluvalserglyleuthraspleucysgluileglugluleulys

770775780

serlyslysleualaleualaprocysasnglnalaglymetglyval

785790795800

valglylysvalglygluileglncysserserglugluseralaarg

805810815

serilelyslysaspglycysiletrpasnalaaspleuvalglyile

820825830

gluleuargvalaspaspalavalcystyrserlysilethrserval

835840845

glualavalalaasntyrseralaileprothrthrileglyglyleu

850855860

argphegluargserhisaspserglnglylysileserglyserpro

865870875880

leuaspilethralaileargglyserpheservalasntyrarggly

885890895

leuargleuserleusergluilethralathrcysthrglygluval

900905910

thrasnvalserglycystyrsercysmetthrglyalalysvalser

915920925

ilelysleuhisserserlysasnserthralahisvalargcyslys

930935940

glyaspgluthralapheservalleugluglyvalhissertyrthr

945950955960

valserleuserpheasphisalavalvalaspgluglncysglnleu

965970975

asncysglyglyhisgluserglnvalthrleulysglyasnleuile

980985990

pheleuaspvalprolysphevalaspglysertyrmetglnthrtyr

99510001005

hisserservalprothrglyalaasnileproserprothrasptrp

101010151020

leuasnalaleupheglyasnglyleuserargtrpileleuglyval

1025103010351040

ileglyvalleuleuglyglyleualaleuphepheleuilemetser

104510501055

leuphelysleuglythrlysglnilepheargserargthrlysleu

106010651070

ala***

<210>23

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<212>prt

<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3np

<400>23

metserglutrpserargilealavalglupheglygluglnglnleu

151015

asnleuthrgluleugluaspphealaarggluleualatyrglugly

202530

leuaspproalaleuileilelyslysleulysgluthrglyglyasp

354045

asptrpvalargaspthrlyspheileilevalphealaleuthrarg

505560

glyasnlysilevallysalaserglylysmetserasnserglyser

65707580

lysargleumetalaleuglnglulystyrglyleuvalgluargala

859095

gluthrargleuserilethrprovalargvalalaglnserleupro

100105110

thrtrpthrcysalaalaalaalaalaleulysglutyrleuproval

115120125

glyproalavalmetasnleulysvalgluasntyrproproglumet

130135140

metcysmetalapheglyserleuileprothralaglyvalserglu

145150155160

alathrthrlysthrleumetglualatyrserleutrpglnaspala

165170175

phethrlysthrileasnvallysmetargglyalaserlysthrglu

180185190

valtyrasnserpheargaspproleuhisalaalavalasnserval

195200205

phepheproasnaspvalargvallystrpleulysalalysglyile

210215220

leuglyproaspglyvalproserargalaalagluvalalaalaala

225230235240

alatyrargasnleu***

245

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<213>未知

<220>

<223>发热伴血小板减少综合征病毒b-3ns

<400>24

metserleuserlyscysserasnvalaspleulysservalalamet

151015

asnalaasnthrvalargleugluproserleuglyglutyrprothr

202530

leuarglysaspleuvalglucyssercysservalleuthrleuser

354045

metvallysargmetglylysmetthrasnthrvaltrpleuphegly

505560

asnprolysasnproleuhisglnleugluproglyleugluglnleu

65707580

leuaspmettyrtyrlysaspmetargcystyrserglnarggluleu

859095

seralaleuargtrpproserglylysproservaltrppheleugln

100105110

alaalahismetphepheserilelysasnsertrpalametgluthr

115120125

glyarggluasntrpargglyleuphehisargilethrlysglylys

130135140

lystyrleuphegluglyaspmetileleuaspserleuglualaile

145150155160

glulysargargleuargleuglyleuprogluileleuilethrgly

165170175

leuserproileleuaspvalalaleuleuglnilegluserleuala

180185190

argleuargglymetserleuasnhishisleuphethrserserser

195200205

leuarglysproleuleuaspcystrpaspphepheileproilearg

210215220

lyslyslysthraspglysertyrservalleuaspgluaspaspglu

225230235240

proglyvalleuglnglytyrprotyrleumetalahistyrleuasn

245250255

argcysprophehisasnleuileargpheaspglugluleuargthr

260265270

alaalaleuasnthriletrpglyargasptrpproalaileglyasp

275280285

leuprolysgluval***

290

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